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Plasmid#207467PurposeLentiviral expression of VGAT-pHluorinDepositorArticleInsertVGAT (Slc6a12 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Sec22b-shR
Plasmid#208358PurposeMammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22bDepositorInsertSec22b (Sec22b Rat)
UseTagsEGFPExpressionMammalianMutationfour silent mutations introduced to render shRNA …PromoterCMVAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C
Plasmid#217983Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172CPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-LC3
Plasmid#221738PurposeStable expression of mRuby3-LC3 in mammalian cellsDepositorInsertLC3B (Map1lc3b Rat)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
icLCT
Plasmid#223179PurposeNative rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)DepositorInsertcytosolic intestinal lactase protein fragment (Lct Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac HTB His-TEV-rDPP9(S729A)-FLAG
Plasmid#217329PurposePurification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9-FLAG
Plasmid#217328PurposePurification of rat DPP9 fusion with C-terminal FLAG tag from insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)
Plasmid#217327PurposePurification of catalytically-dead rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9
Plasmid#217326PurposePurification of rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG
Plasmid#217325PurposePurification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationN61KPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1-FLAG
Plasmid#217323PurposePurification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG
Plasmid#217321PurposePurification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-LEW-rNLRP1-FLAG
Plasmid#217320PurposePurification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP SV2A GFP
Plasmid#218544PurposeMamallian Expression of SV2A GFPDepositorInsertSV2A
UseTagsExpressionMammalianMutationS42FPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-LAP2beta
Plasmid#222416PurposeTo express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLAP2beta (Tmpo Rat)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGK mScarlet-Synaptophysin
Plasmid#206145PurposePGK mScarlet-SynaptophysinDepositorInsertsynaptophysin (Syp Rat)
UseTagsmScarletExpressionMammalianMutationPromoterPGKAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV HA-RIM2
Plasmid#206151PurposeCMV HA-RIM2DepositorInsertRim2 (Rims2 Rat)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -