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pGEM-myr-Citrine-SV40pA-FKF

Plasmid

#119865

Purpose

Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

Insert

myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

2x myristoylation

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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mdm1_pJet1.2

Plasmid

#121977

Purpose

for generating mdm1 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

Insert

mdm1 (mdm1 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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zebrafish SLC2A2 (zfSLC2A2)

Plasmid

#65103

Purpose

Full length SLC2A2 cDNA (Danio rerio)

Depositor

Josep Planas

Article

Castillo et al Am J Physiol Regul Integr Comp Physiol.

Insert

SLC2A2 (slc2a2 Zebrafish)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
Rab43 cds in pGem-T Easy

Plasmid

#80565

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab43 (rab43 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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Rab20 cds in pGem-T Easy

Plasmid

#80540

Purpose

Can be used for in situ hybridisation or other purposes

Depositor

Rob Parton

Article

Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

Insert

rab20 (rab20 Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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miR-223-RFP pCDNA

Plasmid

#97142

Purpose

expression of miR-223 and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Tags

TagRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Cul1a-3'UTR

Plasmid

#97152

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Cul1a 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Cul1b-3'UTR

Plasmid

#97153

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Cul1b 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Traf6-3'UTR

Plasmid

#97154

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Traf6 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod2 guide

Plasmid

#107604

Purpose

An entry vector with U6a and U6c promoter driving sod2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod2 guide RNA sequences

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-sod1 guide

Plasmid

#107603

Purpose

An entry vector with U6a and U6c promoter driving sod1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

sod1 guide RNA sequences

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-uqcrc1 guide

Plasmid

#107602

Purpose

An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

uqcrc1 guide RNA sequences

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-ndufs2 guide

Plasmid

#107601

Purpose

An entry vector with U6a and U6c promoter driving ndufs2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

ndufs2 guide RNA sequences

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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Pet28a His6-HSPB7 (zebrafish) C49S

Plasmid

#110071

Purpose

Express C49S mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Use

Tags

His6

Expression

Bacterial

Mutation

Cysteine 49 to Serine

Promoter

T7

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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