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Plasmid#170349PurposemT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Fascin-C-10
Plasmid#57376PurposeLocalization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFascin (FSCN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PINK1 gRNA (BRDN0001145357)
Plasmid#78036Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorInsertPINK1 (PINK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV10-3xFlag Skp2 WT
Plasmid#81115Purposemammalian expression of Skp2 WTDepositorInsertSKP2 (SKP2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-TRE-mCherry-attB53
Plasmid#183611PurposeRMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInsertTRE-mCherry
UseRmceTagsExpressionMutationPromoterTREAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGExpressionMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Gap43
Plasmid#74336PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.DepositorInsertgrowth associated protein 43 (Gap43 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMS82
Plasmid#68110PurposeIntegration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replicationDepositorInsertsaac3(IV)
Hyg
oriT
attP
int
UseStreptomyces integrating vectorTagsExpressionMutationFragmentPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Man2a1-GFP
Plasmid#163649PurposeExpresses GFP tagged Man2a1 in mammalian cellsDepositorInsertmannosidase alpha class 2A member 1 (MAN2A1 Human)
UseTagsGFPExpressionMammalianMutationSilent mutation was made to introduce a XbaI rest…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only