Addgene

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mEmerald-LaminB1-10

Plasmid

#54140

Purpose

Localization: Nuclear Envelope, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-tdMCP-24xGCN4

Plasmid

#205157

Purpose

express tdMCP-24xGCN4

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-24xGCN4

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2700

Plasmid

#142978

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ESRRG (ESRRG Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-TVA-eGFP-N2cG

Plasmid

#175439

Purpose

Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry_gRNA

Plasmid

#80457

Purpose

gRNA expression vector with mCherry transfection control

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Inserts

sp Cas9 gRNA
mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin promoter

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Use

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Use

Tags

SMASh tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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