-
Plasmid#118500Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)
Plasmid#70211Purpose1 Part of 4-part switchDepositorInsertdeCFP
UseTagsExpressionMutationPromoterAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS
Plasmid#191368PurposeOverexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cellsDepositorInsertNLS-NLS-dHgm4Cas13b-3xGFP-NLS
UseTags3xGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sm-Myocardin Omni-pShuttle
Plasmid#122122PurposeExpression of the smooth muscle version of myocardinDepositorInsertMyocardin (Myocd Mouse)
UseTagsOmniExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pLPC
Plasmid#73560Purposevector encoding 3 Flag tagsDepositorInsert3X-Flag
UseRetroviralTags3X-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-22
Plasmid#120001PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P14
Plasmid#120025PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5DepositorInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSSM97: pCMV-HA-NLS-SpuFz1-E498R
Plasmid#205275PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-E498RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationE498RPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM105: pCMV-HA-NLS-SpuFz1-T513K
Plasmid#205276PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-T513KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationT513KPromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM58: pCMV-HA-NLS-SpuFz1-D487K
Plasmid#205274PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D487KDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD487KPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM59: pCMV-HA-NLS-SpuFz1-D300R
Plasmid#205272PurposeHuman expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300RDepositorInsertSpuFz1
UseTagsHA, NLS(Makkerh)ExpressionMammalianMutationD300RPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-vioABECD
Plasmid#66535PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoterDepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterConsensus T7Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-tdKatushka2-WPRE
Plasmid#193337PurposeExpresses tdKatushka2 under the TRE promoter in mammalian cellsDepositorInserttdKatushka2
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-deltaR8.2(A14C,E45C,A92E)
Plasmid#79048Purposemammalian expression, lentiviral packagingDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P33
Plasmid#120018PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN G129E
Plasmid#50523DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationG129EPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P10
Plasmid#120021PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-11
Plasmid#119990PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm4Cas13b-EGFP-NLS
Plasmid#191360PurposeOverexpression of NLS-dHgm4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm4Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z15
Plasmid#120058PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P24
Plasmid#120035PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAPDepositorInsertTAP
UsePcr templateTagsTAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rhoA WT
Plasmid#61631Purposemammalian expression of rhoADepositorInsertrhoA (RHOA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179070PurposeVector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondriaDepositorInsertRPL18Bp-preCOX4-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)
Plasmid#179064PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuoleDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VAM3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)
Plasmid#179052PurposeVector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleusDepositorInsertRPL18Bp-SRM1-Link-Scarlet(rfp)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Skp2 WT
Plasmid#81117Purposebacterial expression plasmid of Skp2DepositorInsertSKP2 (SKP2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSM12
Plasmid#101643PurposeEncodes the fluorescent protein meffCFP for constitutive expression in E. coli.DepositorInsertmeffCFP
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmp15
Plasmid#163594PurposeExpresses BMP-15 in mammalian cellsDepositorInsertBmp15 (Bmp15 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HOXC13(human)-pET
Plasmid#8679DepositorInsertHOXC13 (HOXC13 Human)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by Coomassie blue stainingPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P15
Plasmid#120026PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
sm-9xGCN4-Renilla-MS2
Plasmid#178009PurposeExpresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cellsDepositorInsertsm-9xGCN4
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RCK
Plasmid#25065DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationContains only the ROC-COR-Kinase domains aa 1329…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCD4 e/p Sal-(hCD2)
Plasmid#24070DepositorInsertCD2 (CD2 Human)
UseTagsmCD 4 promoterExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMJA179
Plasmid#128537PurposeExpresses anti-Prunus dulcis scFv AbDepositorInsertscFv Ab clone PD1F6
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN2AG5kwh
Plasmid#128253PurposeMammalian linearizer gene circuit in a Flp-In expression system based on negative feedback. The hTetR and EGFP is separated by a P2A motif.DepositorInsertBeta Globin Intron::hTetR::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pr-deGFP-MGapt
Plasmid#67734PurposeOR2-OR1-Pr promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T500
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-06
Plasmid#119985PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry
Plasmid#114726PurposedCas9-VP160 expression in mammalian cellsDepositorInsertdCas9-VP160
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-NAD
Plasmid#193824PurposeCMV overexpression of ChemoB-NAD in mammalian cell linesDepositorInsertChemoB-NAD
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(1)
Plasmid#166984PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEV5_ RpMatB_2
Plasmid#78206PurposeExpression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter bindingDepositorInsertmatB (His6 C106A S170A R286C Q457C K488A)
UseTagsHis6ExpressionBacterialMutationC106A S170A R286C Q457C K488APromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-600n
Plasmid#13541DepositorInsertFLIP-E WT
UseTagsECFP and VenusExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z08
Plasmid#120051PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P18
Plasmid#120029PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5DepositorInsertmKeima8.5
UsePcr templateTagsmKeima8.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSU6-shScr/CMV-eGFP
Plasmid#89912PurposeExpress shRNA Scrambled controlDepositorInsertshRNA Scrambled
UseTagseGFP under separate promoterExpressionMammalianMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-17
Plasmid#119996PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
Plasmid#183069PurposeBacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.DepositorInsertCas12c_4
UseCRISPRTagsHis10 and MBPExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT sfGFP 150TAG
Plasmid#154767Purposeplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAGPromoterEF1Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only