pBSU6-shScr/CMV-eGFP
(Plasmid
#89912)
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PurposeExpress shRNA Scrambled control
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 89912 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBSU6
- Backbone size w/o insert (bp) 3292
- Total vector size (bp) 4972
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshRNA Scrambled
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gRNA/shRNA sequenceATTAAGATTGGCGGAATAG
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SpeciesM. musculus (mouse)
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Tag
/ Fusion Protein
- eGFP under separate promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (destroyed during cloning)
- 3′ cloning site SbfI (destroyed during cloning)
- 5′ sequencing primer GTAAAACGACGGCCAGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSU6-shScr/CMV-eGFP was a gift from Robert Friedlander (Addgene plasmid # 89912 ; http://n2t.net/addgene:89912 ; RRID:Addgene_89912) -
For your References section:
Inhibition of mitochondrial protein import by mutant huntingtin. Yano H, Baranov SV, Baranova OV, Kim J, Pan Y, Yablonska S, Carlisle DL, Ferrante RJ, Kim AH, Friedlander RM. Nat Neurosci. 2014 Jun;17(6):822-31. doi: 10.1038/nn.3721. Epub 2014 May 18. 10.1038/nn.3721 PubMed 24836077
Map uploaded by the depositor.
