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Plasmid#108886PurposeExpression of membrane localized mCherryDepositorInsertmCherry-CaaX(Hras)
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SRPK2
Plasmid#23766DepositorInsertSRPK2 (SRPK2 Human)
UseGateway donor vectorTagsExpressionMutation1MMPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA 66L
Plasmid#19300DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationQ66LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
PARP1 FL
Plasmid#169815PurposeExpresses codon optimised full length His tagged PARP1 in bacterial cellsDepositorInsertPoly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)
UseTagsMKHHHHHHMKQExpressionBacterialMutationValine 762 mutated to an alaninePromoterT7 promoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB2G
Plasmid#195290PurposeBicistronic HSV-TK and mTagBFP resistance for positive/negative selectionDepositorInsertHSV-TK 2A mTagBFP
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HP1a-mCherry
Plasmid#185012PurposeBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion ProteinDepositorInsertCBX5 (HP1a) (Cbx5 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2
Plasmid#174817PurposeMammalian expression of SaCas9 prime editor 2DepositorInsertSaPE2
UseTagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2clust
Plasmid#105624PurposeExpresses improved CRY2PHR homo-oligomerizationDepositorInsertmCherry-CRY2PHR-9aa (CRY2 Mustard Weed, Human)
UseTags9 amino acidsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApaLID211A-HA
Plasmid#211459PurposeExpress mitochondrial localized ApaLI with D211A mutationDepositorInsertApaLI-D211A
UseTagsHAExpressionMammalianMutationD211APromoterCMVAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
gamma Synuclein-VC
Plasmid#89734Purposeexpresses human gamma Synuclein fused to the C-terminal part of VenusDepositorInsertSNCG (SNCG Human)
UseTagsC-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mH2A2-CT-GFP
Plasmid#45171DepositorUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG LOC550643 pFCPGW
Plasmid#86853Purposeretroviral expression of NoBodyDepositorInsertLOC550643 (NBDY )
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRF-SrpR
Plasmid#49372DepositorInsertSrpR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CD3 delta YFP
Plasmid#11951DepositorInsertCD3delta-YFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMIR-FLAG-MLL-AF9
Plasmid#71444PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2DepositorInsertMLL-AF9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-hGRK4
Plasmid#32690DepositorInsertGRK4 (GRK4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pD434-SR mouse Alpha-synuclein
Plasmid#89073Purposebacterial expression of mouse Alpha-synucleinDepositorInsertAlpha-synuclein (Snca Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTurquoise2-Paxillin
Plasmid#176107PurposeFocal Adhesion markerDepositorInsertPaxillin (PXN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - EGFP sgRNA 5
Plasmid#51764PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsCas9
Puromycin resistance
EGFP sgRNA 5
UseCRISPR and LentiviralTagsExpressionMutationPromoterEFS and U6Available sinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCRISPRed pRSETB
Plasmid#159454PurposeExpresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRedDepositorInsertmCRISPRed
UseTags10 x His-TagExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHD422N
Plasmid#194432PurposeAPOBEC1 fused to YTHD422NDepositorInsertAPOBEC1-YTHD422N (APOBEC1 Human)
UseCRISPRTagsAPOBEC1-YTHD422N-HAExpressionMammalianMutationD422N mutation in the YTH domain to enhance m6A b…PromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS185 CMV-Cre
Plasmid#11916PurposeMammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoterDepositorInsertcre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-MCPA2-C-18
Plasmid#57469PurposeLocalization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580DepositorInsertMCPA2 (Capza2 Mouse)
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNCS
Plasmid#17362DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR
Plasmid#207878PurposeAAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV enAsCas12f-YHAM-sgRNA_v7
Plasmid#208356PurposeExpression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cellsDepositorInsertenAsCas12f-YHAM
UseTagsExpressionMammalianMutationF48Y/S188H/V232A/E316MPromoterCMVAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA 21L
Plasmid#19299DepositorInsertRas-related GTP binding A (RRAGA Human)
UseTagsGST and HAExpressionMammalianMutationT21LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorUseTagsExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Tol2-PA2-CMV-AB-mCh
Plasmid#160435PurposeExpresses human Amyloid Beta-mCherry in ZebrafishDepositorInsertHuman Amyloid Beta peptide (1-42) (APP Human, Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterCMVAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-WT-STAT1
Plasmid#71454PurposeLentiviral expression of WT-STAT1DepositorInsertSTAT1 (STAT1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMVAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNS35 (multiAsCas12a piggyBac)
Plasmid#217336PurposepiggyBac expression of multiAsCas12aDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBJ 2358
Plasmid#89245PurposeExpresses YFP fused to the N-terminus of Mouse Capping Protein Alpha1, using the Clontech vector PEYFP C-1.DepositorInsertCapza1 (Capza1 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9
Plasmid#201949PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en31FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationG1243T, E1369R, E1603H on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF.mHES1.CMV.GFP
Plasmid#17622DepositorInsertHES1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB304
Plasmid#68632PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGUSExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mTagBFP2-WPRE
Plasmid#193339PurposeAAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseAAVTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
R6K_tac_mCherry_ChInt
Plasmid#187382Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr261-EGFP
Plasmid#58471PurposeExpresses a cytoplasmic actin filament reporter, the tandem CH domains (a.a. 1-261) from human utrophin, on a CMV promoterDepositorInsertUtr261 (UTRN Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSup
Plasmid#149416PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-alpha-E-Catenin 3xFP4 (895)
Plasmid#67936PurposeMammalian expression of alpha E cateninDepositorInsertalpha E catenin (Ctnna1 Mouse)
UseTags3xFP4 and EGFPExpressionMammalianMutationN-terminal domainPromoterCMVAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MyoD-pCLBabe
Plasmid#20917DepositorInsertMyoD (Myod1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTETRIS-cargo
Plasmid#126033PurposeLuciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.DepositorTypeEmpty backboneUseLuciferase; PiggybacTagsExpressionMammalianMutationPromoterTREAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PresentER-Cassette GFP
Plasmid#102942PurposePresentER with removable cassette for cloning of MHC-I antigen (GFP version)DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean3
Plasmid#103973PurposePlasmid encoding mCerulean3DepositorInsertmCerulean3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorUseTagsExpressionBacterialMutation0PromoterAmpRAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only