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pF CAG luc-EGFP-cre puro

Plasmid

#67503

Purpose

Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

luciferase-EGFP-cre

Use

Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-LSL-mCherry-shPtbp1

Plasmid

#178591

Purpose

Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Ptbp1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Dest-pA-pgk-bsd

Plasmid

#74918

Purpose

Gateway destination piggyBac vector

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
pAAV2/1-CAG-Flex-FlpO-WPRE

Plasmid

#171690

Purpose

Cre-dependent expression of Flp-recombinase

Depositor

Mark Huebener, Tobias Bonhoeffer, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

Flip-recombinase

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

Plasmid

#114111

Purpose

fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

Inserts

Gaussia luciferase, slow burn
Guillardia theta anion channelrhodopsin-2, human codon optimized
Enhanced Yellow Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_3

Plasmid

#178049

Purpose

CAG tdGFP Lrig1 shRNAmir-3 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_2

Plasmid

#178048

Purpose

CAG tdGFP Lrig1 shRNAmir-2 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-L-bpA-2xSV40pA-L-tdGFP-Lrig1_shRNAmir_1

Plasmid

#178047

Purpose

CAG tdGFP Lrig1 shRNAmir-1 cre/lox expression piggyBac transposon (bpA 2xSV40pA stop)

Depositor

Mario Capecchi

Insert

Lrig1 shRNAmir

Use

Cre/Lox and RNAi; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CAG-mRuby3-Gal8-P2A-Zeo

Plasmid

#150815

Purpose

Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

Insert

mRuby3-Gal8-P2A-Zeo (LGALS8 Human, Synthetic)

Use

Tags

Gal8 is fused to the c-terminus of mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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lenti CAG-FLAG-dCas9-VPR

Plasmid

#114197

Purpose

Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CAG-empty-ires-EGFP

Plasmid

#122953

Purpose

Lentiviral expression empty vector under CAG promoter with EGFP expression

Depositor

Pierre Vanderhaeghen

Article

Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 BQ.1.1 Spike

Plasmid

#193710

Purpose

Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

Depositor

Marceline Côté

Article

Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

Insert

SARS-CoV-2 BQ.1.1 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19I, LPPA24S, …

Promoter

chicken β-actin promoter, CMV enhancer

Available since

Nov. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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PD2529-CAG-Flag-mouse TGFb2

Plasmid

#180261

Purpose

Mouse full length TGFβ2 expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

TGFβ2 (Tgfb2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Brainbow3.2-PGK-PuroTK

Plasmid

#159928

Purpose

Brainbow transgenesis

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

Inserts

NFPnls
mOrange2
EGFP
mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 5HT3 LC IRES GFP

Plasmid

#123066

Purpose

chemogenetics

Depositor

Scott Sternson

Article

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Insert

PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)

Use

Tags

Expression

Mammalian

Mutation

L131G Q139L Y217F

Promoter

CAG

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-Flag-mouse TGFb3

Plasmid

#180262

Purpose

Mouse full length TGFβ3 expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

TGFβ3 (Tgfb3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Delta Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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