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Plasmid#75693Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K7DepositorInsertMAP3K7 (MAP3K7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K7 gRNA (BRDN0001148226)
Plasmid#75694Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K7DepositorInsertMAP3K7 (MAP3K7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BMPR1B_p.Q203D
Plasmid#82173PurposeGateway Donor vector containing BMPR1B , part of the Target Accelerator Plasmid Collection.DepositorInsertBMPR1B (BMPR1B Human)
UseGateway entry vectorTagsExpressionMutationQ203DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX311-GFP-MEKDD
Plasmid#194882PurposeExpression of dominant negative MEK1DepositorInsertMEKDD (MAP2K1 Human)
UseLentiviralTagsExpressionMammalianMutationS218D, S222DPromoterE1FaAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hLAMP2-C-GC6s
Plasmid#154151PurposeTo detect the calcium ion releases close to the lysosomal surfaceDepositorInsertLAMP2 and GCamp6s (LAMP2 Human)
UseLentiviralTagsExpressionMutationWTPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai3-RLuc8
Plasmid#140975PurposeEncodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai3-RLuc8 (GNAI3 Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-LRIG1-COMP5AP-AviTag-9xHis
Plasmid#157538PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertLRIG1 (LRIG1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP HAT
Plasmid#179547Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-p21-HA
Plasmid#215110PurposeExpresses p21-HA in mammalian cells from an inducible lentiviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMV (Tet-inducible)Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NTRK1
Plasmid#23891DepositorInsertNTRK1 (NTRK1 Human)
UseGateway donor vectorTagsExpressionMutationV263L relative to NM_001012331PromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
p21-mCerulean-NeoR
Plasmid#215080PurposeExpresses p21-mCerulean in mammalian cells.DepositorInsertCDKN1A (CDKN1A Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Myc-FBW7 Delta F-Box
Plasmid#197452PurposeThe plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.DepositorInsertFBW7 (FBXW7 Human)
UseTagsMycExpressionMammalianMutationFBW7 Fbox deleted.PromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CREBBP_ZNF384
Plasmid#205796PurposeExpress mEGFP-tagged fusion protein, CREBBP_ZNF384 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc-mCh-Tub
Plasmid#24288DepositorInsertAlpha-Tubulin (alphaTub84B Fly)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-JAG1-E228K
Plasmid#116420PurposeLentiviral expression of JAG1 E228KDepositorInsertJAG1 (JAG1 Human)
UseLentiviralTagsExpressionMutationE228KPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CDK17_WT
Plasmid#81776PurposeGateway Donor vector containing CDK17 , part of the Target Accelerator Plasmid Collection.DepositorInsertCDK17 (CDK17 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CSF2RA_CRLF2
Plasmid#205798PurposeExpress mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PKDCC
Plasmid#23387DepositorInsertPKDCC (PKDCC Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CDKN1A_WT
Plasmid#82201PurposeGateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.DepositorInsertCDKN1A (CDKN1A Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7_R505C
Plasmid#160104PurposeExpresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cellsDepositorInsertFbxw7 alpha R505C (Fbxw7 Mouse)
UseLentiviralTagsExpressionMammalianMutationR505CPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only