CL20_mEGFP_CSF2RA_CRLF2
(Plasmid
#205798)
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PurposeExpress mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneCL20
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCSF2RA_CRLF2
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SpeciesH. sapiens (human)
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Entrez GeneCSF2RA (a.k.a. CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMCSFR-alpha, GMR, GMR-alpha, SMDP4, alphaGMR)
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Entrez GeneCRLF2 (a.k.a. CRL2, CRLF2Y, TSLPR)
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CL20_mEGFP_CSF2RA_CRLF2 was a gift from Richard Kriwacki (Addgene plasmid # 205798 ; http://n2t.net/addgene:205798 ; RRID:Addgene_205798) -
For your References section:
Defining the condensate landscape of fusion oncoproteins. Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG, Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni N, Kriwacki RW. Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2. 10.1038/s41467-023-41655-2 PubMed 37770423