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Plasmid#44353DepositorInsertEF1a-CHER
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2526-3969)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hNPPC-RFP
Plasmid#22921DepositorInserthuman c-type natruiretic peptide precursor promoter driving DsRedExpress (NPPC Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_BpiI-cloning-site_SV40polyA
Plasmid#124547PurposeDestination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL758
Plasmid#219504PurposeExpress Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequencesDepositorInsertmsr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
UseTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-himar-apraR
Plasmid#199995PurposeLanding Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationhimar frameshiftPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed mRuby3 VLP
Plasmid#182431PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
deltaVP1
VP2C-mRuby3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-R4-bpFOG-L5
Plasmid#186369PurposeEntry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequencesDepositorInsertBasal promoter of the Friend Of Gata gene
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT048A
Plasmid#159409PurposeConstitutive LuxR expression, controlled under J23101 E.coli promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23101-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromoterJ23101 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT046A
Plasmid#159407PurposeConstitutive LuxR expression, controlled under E.coli tet promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.tet-LuxR-pr.LuxR-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_araBAD
Plasmid#155172PurposeBaSP and CuCBP co-expression: araBAD promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_lacZ
Plasmid#155169PurposeBaSP and CuCBP co-expression: lacZ promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL052
Plasmid#137906PurposeOsBiP1 promoter driven Cas9-tRNA systemDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL053
Plasmid#137907PurposeOsBiP1 promoter driven Cas9-csy4 systemDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only