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Plasmid#147819PurposeMammalian Expression of HsSmg5-G120EDepositorInsertHsSmg5-G120E (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_633-1091_T
Plasmid#147558PurposeMammalian Expression of HsSmg7_633-1091DepositorInsertHsSmg7_633-1091 (SMG7 Human)
UseTagsExpressionMammalianMutationone non silent mutation V854I compared to the seq…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD2
Plasmid#180602PurposeExpressing various parts of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-myc-TRBP-dsRBD1
Plasmid#180601PurposeExpressing various parts of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Arg57Ala
Plasmid#180759PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)DepositorInsertα-COPI-WD40-Arg57Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Arg57Ala, Leu1…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Tyr139Ala
Plasmid#180761PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)DepositorInsertα-COPI-WD40-Tyr139Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Tyr139Ala, Leu…PromoterCMVAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40-Asp115Ala
Plasmid#180760PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)DepositorInsertα-COPI-WD40-Asp115Ala
UseTagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Asp115Ala, Leu…PromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRBP-L2+D2+L3-myc/His
Plasmid#180600PurposeExpressing various parts of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBIV
Plasmid#176055PurposeExpresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 304-324PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBIIb
Plasmid#176054PurposeExpresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 259-270PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBIIa
Plasmid#176053PurposeExpresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 188-199PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBII
Plasmid#176052PurposeExpresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 128-143PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMBI
Plasmid#176051PurposeExpresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 44-63PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-V5 Myc dMB0
Plasmid#176050PurposeExpresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cellsDepositorInsertMyc
UseTags6xHis and V5ExpressionMammalianMutationdeleted amino acids 16-33PromoterCMVAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-PRGRH-Nluc
Plasmid#158608PurposePlasmid expressing PRG(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-p115RH-Nluc
Plasmid#158607PurposePlasmid expressing p115(RH)-Nluc in mammalian cellsDepositorInsertmyristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA USP7
Plasmid#131253PurposeMammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationContains point mutations that convert USP7 aspart…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA 1-205 USP7
Plasmid#131254PurposeMammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tagDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-2…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only