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Showing: 1581 - 1600 of 5212 results

  5. pScRad52-SpCas9 (pXK-1646)

    Plasmid
    #208823
    Purpose
    CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.
    Depositor
    Kun Xu
    Article
    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-
    Type
    Empty backbone
    Use
    CRISPR; Cas9
    Tags
    Rad52
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    208823-plasmid-map-sequence-id-417236

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  6. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  7. pCAG-GFP-PQRv3-RFP

    Plasmid
    #73951
    Purpose
    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.
    Depositor
    Brian Chen
    Article
    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do
    Inserts
    sfGFP (superfolder GFP)
    RFP
    Use
    Tags
    A residual PQR peptide wii be left at the C-termi…
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin and Chicken beta actin (shared…
    Available since
    Jan. 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. pCAG-attBr-ZsGreen-attP

    Plasmid
    #51265
    Purpose
    Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    attBr-ZsGreen-attP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51265-plasmid-map-sequence-id-77875

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  12. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  14. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid
    #159363
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.
    Depositor
    Michael Farzan, Hyeryun Choe
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Use
    Tags
    Flag and Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159363-plasmid-map-sequence-id-310910

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  15. pCAG-attBr-Cre-rc-attP

    Plasmid
    #51264
    Purpose
    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    attBr-Cre-rc-attP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51264-plasmid-map-sequence-id-77874

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  17. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Michael Farzan, Hyeryun Choe
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156419-plasmid-map-sequence-id-308855

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Showing: 1581 - 1600 of 5212 results