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pAB035h

Plasmid

#79191

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-HA4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTXRE5-Z

Plasmid

#112509

Purpose

Reporter plasmid, responsive to activated Ah receptor/Arnt

Depositor

Charles Miller

Article

Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3

Insert

RW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)

Use

Tags

Expression

Yeast

Mutation

Promoter

5 XREs+ minimal Cytochrome C

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pV132-vCas9

Plasmid

#203223

Purpose

Mammalian expression of vCas9

Depositor

Phil Sharp, Vikash Chauhan, Robert Langer

Article

Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

Insert

vCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

S55R R976A K1003A T1314R

Promoter

CBh

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101102

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

TMt-dCas9(C)-VP64

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAB064d

Plasmid

#79197

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 luxAB; Ppro1 434cI-SH2ABL1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ppro1

Available since

Dec. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_NT135

Plasmid

#183118

Purpose

pLentiGuide modified to express GFP and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-flag Mst2 (KR)

Plasmid

#27372

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mst2 (STK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

K56R (catalytically inactive)

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-PBD-EYFP

Plasmid

#13723

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD-EYFP (PAK1 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

p21 binding domain of human Pak1(amino acids 65-1…

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt11

Plasmid

#35922

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt11 (wnt11f2 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf3(WT)-HA

Plasmid

#79414

Purpose

Expresses C-terminally HA-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (ARF3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNFIL-3

Plasmid

#34572

Depositor

Peter Murray

Article

Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

Insert

NFIL-3 (Nfil3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S109A

Plasmid

#33088

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S109A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET21b GFP-Rab10 Q68LHis

Plasmid

#186015

Purpose

GFP-Rab10 Q68LHis

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

GFP-Rab10 Q68LHis (RAB10 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-OAZ

Plasmid

#14939

Depositor

Joan Massague

Article

Hata et al Cell. 2000 Jan 21. 100(2):229-40.

Insert

OAZ (ZNF423 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-Gal4-Vp16-Crys-CFP

Plasmid

#97144

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Gal4-Vp16

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits