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Plasmid#58879PurposeMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV proteaseDepositorInsertMESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCGB
Plasmid#135283PurposeGBPart - CGB Luciferase CDSDepositorInsertClick Beetle Green Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP_SCF2_LD6_NTEV
Plasmid#58878PurposeMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV proteaseDepositorInsertMESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETAipi
Plasmid#53284PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coliDepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx1(yeast)
Plasmid#65004PurposeYeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1DepositorInsertGlutaredoxin-1
UseTagsroGFP2ExpressionYeastMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE MKL1-N100
Plasmid#19848DepositorInsertMKL1-N100 (MRTFA Human)
UseRetroviralTags3xFLAGExpressionMutationdeleted amino acids 1-100PromoterAvailable sinceJan. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu8
Plasmid#98297PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Omega 2
Plasmid#118047PurposeGoldenBraid2.0 Omega 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Alpha2
Plasmid#118045PurposeGoldenBraid2.0 Alpha 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…TagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFAP-Arclight
Plasmid#187978PurposeExpresses the fluorescent voltage indicator Arclight under the GFAP promoterDepositorInsertArclight A242
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PBD-EYFP
Plasmid#13723DepositorInsertPBD-EYFP (PAK1 Human)
UseTagsEYFPExpressionMammalianMutationp21 binding domain of human Pak1(amino acids 65-1…PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
Plasmid#45574PurposeExpresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backboneDepositorInsertco I-Sce I
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
phCMV-IE1
Plasmid#118048PurposeGBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cellsDepositorInsertCytomegalovirus enhancer and promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
SeeSaw Reporter (SSR1.0)
Plasmid#50392PurposeSSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.DepositorInsertSSR1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB082b
Plasmid#79234PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d3
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB082h
Plasmid#79240PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d23C
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLuc
Plasmid#135288PurposeGBPart - GLuc Luciferase CDSDepositorInsertGaussia Luciferase
UseLuciferase and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only