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pRK5-FLAG-S6K1

Plasmid

#100509

Purpose

Expresses FLAG tagged S6K1 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pAS_4xMx PylT_FLAG-Mx PylRS

Plasmid

#140011

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLRC1-NEK10p:NEK10-3XFLAG

Plasmid

#137030

Purpose

Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

Depositor

David Sabatini

Article

Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

Insert

NIMA-like kinase 10 (NEK10 Human)

Use

Lentiviral

Tags

3XFLAG

Expression

Mutation

Promoter

human NEK10 endogenous promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C192A

Plasmid

#133891

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2019

Availability

Academic Institutions and Nonprofits only

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3XFlag DVL2 (∂DIX)

Plasmid

#24805

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the N-terminal DIX domain

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1-TAT-2-84-FLAG

Plasmid

#35980

Depositor

Susana Valente

Article

Mousseau et al Cell Host Microbe. 2012 Jul 19;12(1):97-

Insert

transactivating protein (tat )

Use

Tags

Flag

Expression

Mammalian

Mutation

tat from HIV2 84 amino acids

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT C39A

Plasmid

#133887

Purpose

Expresses Gsdmd-NT mutant in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT

Use

Tags

Expression

Mammalian

Mutation

C39A

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xFlag-ERK2

Plasmid

#47573

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged ERK2

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

ERK2 (Mapk1 Rat)

Use

Tags

3xFlag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag hDPY30

Plasmid

#15554

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pQCXIH BRGI-Flag

Plasmid

#19148

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1a2

Plasmid

#13751

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a2 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pPOTv7-hygro-10FLAG

Plasmid

#179815

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

10xFLAG

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL

Plasmid

#24233

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
PRK5-FLAG-Bag6ΔUBL

Plasmid

#61837

Purpose

express N-terminally FLAG tagged human Bag6 without UBL domain

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

Bag6 (BAG6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only
W118-1_Flag-hIRAK1

Plasmid

#180405

Purpose

lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

IRAK1 (IRAK1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Adrm1

Plasmid

#19417

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pEF-TAZ-N-Flag

Plasmid

#19025

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Vector has no SV40 origin

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
pQFlag-USP11 WT puroR

Plasmid

#46747

Purpose

Retroviral vector that expresses Flag-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge