Addgene

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pACEBac1-GCP4

Plasmid

#178070

Purpose

Insect cell expression of GCP4

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP4 (TUBGCP4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBN079

Plasmid

#167960

Purpose

express E. coli E69 (O9a:K30) Wzc with Y718E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y718E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN072

Plasmid

#167953

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN073

Plasmid

#167954

Purpose

express E. coli E69 (O9a:K30) Wzc with Y706E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y706E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y706E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN075

Plasmid

#167956

Purpose

express E. coli E69 (O9a:K30) Wzc with Y708E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y708E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y708E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN076

Plasmid

#167957

Purpose

express E. coli E69 (O9a:K30) Wzc with Y713E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y713E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y713E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN077

Plasmid

#167958

Purpose

express E. coli E69 (O9a:K30) Wzc with Y715E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y715E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y715E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN078

Plasmid

#167959

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E

Depositor

James Naismith

Article

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E

Use

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Ad-mMKP3 S159A S197A-Flag

Plasmid

#131579

Purpose

Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

Depositor

Haiyan Xu

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mutation

changed Serine 159 to Alanine and Serine 197 to A…

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Use

Tags

CCG

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC28

Plasmid

#104693

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC28 (UBC28 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-J23113

Plasmid

#119701

Purpose

Expression of Cas9 with a weak promoter

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA1

Plasmid

#107076

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA3

Plasmid

#107078

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-MsePCNA3

Plasmid

#107075

Purpose

M. sedula PCNA3

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMD-SsoPCNA2

Plasmid

#107077

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA2

Use

Cloning vector

Tags

Expression

Mutation

Promoter

SP6, lac

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b-M3C

Plasmid

#107087

Purpose

M. sedula PCNA3 fused at the N-terminus of CyPet

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

M. sedula PCNA3 fused to CyPet

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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