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  5. pEDA5_GFPmut3_Y66H

    Plasmid
    #80085
    Purpose
    Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.
    Depositor
    Timothy Whitehead
    Article
    Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do
    Insert
    GFPmut3_Y66H
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    changed Tyrosine 66 to Histidine
    Promoter
    proB
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    80085-plasmid-map-sequence-id-197645

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  14. pMSP1E2D1

    Plasmid
    #161606
    Purpose
    Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    Ritchie et al Methods Enzymol. 2009 . 464():211-31.
    Insert
    MSP1E2D1
    Use
    Tags
    7-His
    Expression
    Bacterial
    Mutation
    "extended" MSP1D1; contains repeat of h…
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161606-plasmid-map-sequence-id-318420

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Showing: 1581 - 1600 of 6831 results