160,048 results

1. pHAGE TRE dCas9-VP64

   Plasmid

   #50916

   Purpose

   Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A nuclease-deficient

   Promoter

   TRE

   Available since

   March 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lentiviral-S-tdTomato

   Plasmid

   #112579

   Purpose

   Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

   Depositor

   Jeffrey Rothstein

   Article

   Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

   Insert

   NLS-tdTomato-NES
   

   Use

   Lentiviral

   Tags

   nuclear export sequence and nuclear localization …

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HIV-1 LTR-gfp

   Plasmid

   #115809

   Purpose

   HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gfp and ngfr
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   HIV1 LTR

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pF CAG luc-EGFP-cre puro

   Plasmid

   #67503

   Purpose

   Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   luciferase-EGFP-cre
   

   Use

   Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe B-Raf V600E

   Plasmid

   #17544

   Depositor

   Channing Der

   Article

   Hao et al Mol Cancer Ther. 2007 Aug . 6(8):2220-9.

   Insert

   B-Raf (BRAF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLH01

   Plasmid

   #117261

   Purpose

   Genome editing for Lactobacillus plantarum WCSF1

   Depositor

   Sheng Yang

   Article

   Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

   Insert

   PorfX-Lp_0640-42, sapK-sappR
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-HA

   Plasmid

   #128034

   Purpose

   Mammalian expression vector with N terminal HA tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMD2.G-VSV-G-mut

    Plasmid

    #196508

    Purpose

    VSV-G mutant (K47Q, R354A)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    VSV-G
    

    Use

    Tags

    NA

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6 asyn WT

    Plasmid

    #107425

    Purpose

    Mammalian expression of human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIF-TRE-mStrawberry reporter

    Plasmid

    #158681

    Purpose

    This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-DIO-EGFP

    Plasmid

    #50457

    Purpose

    Double floxed EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, and AAV8

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-RARE-luciferase

    Plasmid

    #13458

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Retinoic Acid Receptor Response Element
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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