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pEGFP-N1-CaMKKbeta S129A, S133A, S137A

Plasmid

#32454

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

S129A, S133A, S137A

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-mSNX27-H112A RNAi-resistant

Plasmid

#163621

Purpose

mouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

mouse SNX27-H112A RNAi-resistant (Snx27 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

H112A and RNAi resistance

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 R290A D291A T292A

Plasmid

#112103

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R290A D291A T292A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 T292A L293A P294A

Plasmid

#112104

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T292A L293A P294A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 L330R Q334R L702R

Plasmid

#112105

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

L330R Q334R L702R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A F409A

Plasmid

#112108

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R F409A

Plasmid

#112110

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R F409A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 Dynamin1 D406R M407R T488W

Plasmid

#112111

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R T488W

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pF713-pEGFP-N1-Hs-FL-METTL20 wt

Plasmid

#85114

Purpose

transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-VAPA(1-242) K87D/M89D

Plasmid

#18875

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

double point mutant K87D / M89D

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-N1 Dynamin1 T78R L84R T92R V118R

Plasmid

#112106

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T78R L84R T92R V118R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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human EB1 in pEGFP N1 to express EB1-GFP (JB131)

Plasmid

#39299

Depositor

Jennifer Tirnauer, Tim Mitchison

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Use

Tags

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pScarlet-N1

Plasmid

#128060

Purpose

Mammalian expression vector for C terminal mScarlet-I fusions

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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N1-moxDendra2

Plasmid

#89792

Purpose

C-terminal fusion to moxDendra2

Depositor

Erik Snapp

Article

Kaberniuk et al Chem Commun (Camb). 2017 Feb 9;53(13):21

Type

Empty backbone

Use

Tags

moxDendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Use

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyc-N1

Plasmid

#85759

Purpose

A mammalian expression vector with a Myc-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Use

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-U6

Plasmid

#19799

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge