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Addgene

pEGFP-N1-zebrafish lyn(C466A)–GFP
(Plasmid #35957)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35957 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    NA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized zebrafish lyn
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1530
  • Mutation
    C466A
  • GenBank ID
    NP_001004543.1
  • Entrez Gene
    lyn (a.k.a. CH73-38P6.3, zgc:92124)
  • Promoter cmv
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-zebrafish lyn(C466A)–GFP was a gift from Anna Huttenlocher (Addgene plasmid # 35957 ; http://n2t.net/addgene:35957 ; RRID:Addgene_35957)
  • For your References section:

    Lyn is a redox sensor that mediates leukocyte wound attraction in vivo. Yoo SK, Starnes TW, Deng Q, Huttenlocher A. Nature. 2011 Nov 20;480(7375):109-12. doi: 10.1038/nature10632. 10.1038/nature10632 PubMed 22101434