pEGFP-N1-zebrafish lyn–GFP
(Plasmid
#35956)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Modifications to backboneNA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehumanized zebrafish lyn
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1530
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Mutationwt
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GenBank IDNP_001004543.1
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Entrez Genelyn (a.k.a. CH73-38P6.3, zgc:92124)
- Promoter cmv
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe I (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-N1-zebrafish lyn–GFP was a gift from Anna Huttenlocher (Addgene plasmid # 35956 ; http://n2t.net/addgene:35956 ; RRID:Addgene_35956) -
For your References section:
Lyn is a redox sensor that mediates leukocyte wound attraction in vivo. Yoo SK, Starnes TW, Deng Q, Huttenlocher A. Nature. 2011 Nov 20;480(7375):109-12. doi: 10.1038/nature10632. 10.1038/nature10632 PubMed 22101434