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pCRISPaint-TC
Plasmid#67169PurposeCRISPaint universal donor for tetracysteine fusion taggingDepositorInsertTC Tag
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCri-9b
Plasmid#61332PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCri-8b
Plasmid#61331PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-6b
Plasmid#61329PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsGST-tag and His-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCri-9a
Plasmid#61318PurposeProtein expression.DepositorInsertGreen fluorescent protein
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR4-24XPP7SL
Plasmid#31864DepositorInsert24xPP7 Stem Loop Cassette
UseTagsExpressionMutationPromoterAvailable sinceAug. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one
Plasmid#183695PurposeExpresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selectiveDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PE-bacteria
Plasmid#172715PurposeExpression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editingDepositorInsertCas9n-linker-M-MLV2
UseTagsExpressionBacterialMutationH840A of Cas9PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRY2PHR-mCherryN1
Plasmid#26866PurposeExpresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modulesDepositorInsertCRY2PHR (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST
Plasmid#125689PurposeC to T base editor for actinomycetesDepositorInsertstreptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-mcBEST
Plasmid#209416PurposePlasmid for multiplexed cytosine base editing in streptomycetesDepositorInsertsCodon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterbase editing cassette: PtipA ; sgRNAs: PkasO* an…Available sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon
Plasmid#174092PurposeCRISPaint gene taggingDepositorInsertmNeonGreen
UseGene taggingTagsExpressionMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-11b_PcrA_M6_H93A
Plasmid#194156PurposeEngineered PcrA_M6 helicase to support isothermal amplification method SHARPDepositorInsertEngineered helicase PcrA_M6
UseTags6x His tag and FLAG tagExpressionBacterialMutationH93A, C96A, N187C, C247A, L384V, L409CPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-InC
Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR8 JARID2
Plasmid#114443PurposeEntry vector for gateway cloning containing human JARID2 coding sequenceDepositorInsertJARID2 (JARID2 Human)
UseGateway entry cloningTagsExpressionMutationPromoterNoneAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-aBEST
Plasmid#131464PurposeExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetesDepositorInsertsStreptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromotertipAAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
