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Plasmid#212812PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUboExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K63R)-GFP
Plasmid#212722PurposeBacterial expression of His6-CUbo(K63R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K63R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His6-CUbo(K48R)-GFP
Plasmid#212721PurposeBacterial expression of His6-CUbo(K48R)-GFPDepositorInsertGFP
UseTags6xHis-CUbo(K48R)ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-E3(63)-His6
Plasmid#212717PurposeBacterial expression of NUbo-E3(63)-His6DepositorInsertE3(63)
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-NUbo-E3(48)-His6
Plasmid#212714PurposeBacterial expression of NUbo-E3(48)-His6DepositorInsertE3(48)
UseTags6xHis and NUboExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-TEV2-Ubc7-His6
Plasmid#212713PurposeBacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-proteaseDepositorInsertUbc7
UseTags6xHis and MBP-TEV2ExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(31-114)
Plasmid#99514PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(31-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationinternal inversion of nucleotides 265-312 of the …PromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-NUbo-GFP-His6
Plasmid#212790PurposeCopper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeastDepositorInsertNUbo-GFP-His6
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(63)-mCherry-FLAG
Plasmid#212809PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(63)-mCherry-FLAG in mammalian cellsDepositorInsertE3(63)
UseTagsNUbo and mCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
UseTags6xHis and CUbo-TwinStrepExpressionBacterialMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-FLAG
Plasmid#212803PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-FLAG in mammalian cellsDepositorInsertE3(48)
UseTagsNUbo and mCherry-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-VSV
Plasmid#212752PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(63)-VSV
Plasmid#212753PurposeDoxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-TET-NUbo-E3(48)-VSV
Plasmid#212744PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-VSV
Plasmid#212743PurposeDoxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Myc-E3(1) C885A-CUbo
Plasmid#212800PurposeConstitutive/Doxycycline-inducible expression of Myc-E3(1) C885A-CUbo in mammalian cellsDepositorInsertE3(1)
UseTagsCUbo and MycExpressionMammalianMutationHOIP(C885A)PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-His8-H2B-CUbo(K63R)
Plasmid#212814PurposeConstitutive or doxycycline-inducible expression of His8-H2B-CUbo(K63R) in mammalian cellsDepositorInsertH2B
UseTags8xHis and CUbo(K63R)ExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(63)-FLAG
Plasmid#212808PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cellsDepositorInsertE3(63)
UseTagsFLAG and NUboExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His7-NUbo-GFP
Plasmid#212791PurposeCopper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeastDepositorInsertHis7-NUbo-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo-GFP
Plasmid#212792PurposeCopper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeastDepositorInsertHis6-CUbo-GFP
UseIntegrative vectorTagsExpressionYeastMutationPromoterCUP1Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only