-
Plasmid#167622PurposeExpress TonB C18G, V206C.DepositorInserttonB C18G, V206C
UseTagsExpressionBacterialMutationtonB with C18G, V206C mutationsPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1362
Plasmid#167615PurposeExpress TonB C18G.DepositorInserttonB C18G
UseTagsExpressionBacterialMutationtonB with C18G mutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1684
Plasmid#167630PurposeExpress TonB C18G, R214C.DepositorInserttonB C18G, R214C
UseTagsExpressionBacterialMutationtonB with C18G, R214C mutationsPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP476
Plasmid#167594PurposeExpress TonB ∆(199-216)DepositorInserttonB ∆(199-216)
UseTagsExpressionBacterialMutationtonB ∆(199-216)PromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP372
Plasmid#167593PurposeExpress TonB frameshift of amphipathic helixDepositorInserttonB fs
UseTagsExpressionBacterialMutationtonB with frameshift of amphipathic helixPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-rrnOU
Plasmid#112785PurposeUpstream homology arm, rrnO-23S (23S rRNA)DepositorArticleInsertrrnOU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-amyEU
Plasmid#112781PurposeUpstream homology arm, amyE encodes _-amylaseDepositorArticleInsertamyEU
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYH78
Plasmid#217386PurposePtrc99A-PAmCherry for bacterial expressionDepositorInsertPAmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBS.EF/mScarlet(i)-hCdt100Nt
Plasmid#129334PurposeCell cycle reporter, lentivirusDepositorInsertmScarlet(i) (Addgene 85044)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP3665
Plasmid#222351PurposeExpression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_NoMutL_V2_gentR
Plasmid#205293PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_Empty
Plasmid#221480PurposeEmpty vector control for pLX307 overexpresion experimentsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP3653
Plasmid#222348PurposeExpression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen
Plasmid#218645PurposeGateway shuttling vector for APEX2-tagged P4C, a P4C biosensorDepositorInsertSidC(P4C)
UseGateway entryTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUBC-SV40NLS-Pfv-Sapphire-PURO
Plasmid#203654PurposeMammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.DepositorArticleInsertPfV
UseLentiviralTagsSapphireExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_Ec1_V1_gentR
Plasmid#205291PurposeHelper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.DepositorArticleInsertCspRecT / Bxb-1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS403-HIS3-pINO4-SV40NLS-PfV-GS-Sapphire
Plasmid#203658PurposeYeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm GEMs under the HIS3 promoter.DepositorArticleInsertPfV
UseTagsSapphireExpressionYeastMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP3664
Plasmid#222350PurposeExpression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPabstBRAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only