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Plasmid#98845PurposeExpresses Human Dnase1L3 in mammalian cells along with DsRedDepositorInsertDnase1L3 (DNASE1L3 Human)
UseTagsIRES DsRedExpressionMammalianMutationlong isoform, wild typePromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTMVi-N
Plasmid#118756PurposeIntermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.DepositorInsertTobacco mosaic virus cDNA fragment with a cloning polylinker
UseIntermediate to build tobacco mosaic virus recomb…TagsExpressionMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(1363-1370Mut)-MBP
Plasmid#190038PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and MBPExpressionMammalianMutation1363 RDKDLIES 1370 to GSSGGSSGPromoterCMVAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-CPL2/pET28a
Plasmid#172095PurposecDNA of CER11/CPL2 cloned into pET28a for protein expressionDepositorInsertCPL2 (CPL2 Mustard Weed)
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CPL2g/pDONR221
Plasmid#172093PurposeGenomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221DepositorInsertCPL2 (CPL2 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationPromoterCPL2Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZS1[Iq:LacI-T]
Plasmid#60756PurposeContains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.DepositorInsertLacI-T
UseSynthetic BiologyTagsExpressionBacterialMutationLacI without the tetramerization domain and the T…PromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet30b-5M SrtA
Plasmid#51140Purposeexpression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 5M
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-fFuCas9-HTBNLS-hph
Plasmid#121092PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 systemDepositorInsertCas9 and hygromycin resist gene
UseCRISPRTagsExpressionBacterialMutationPromotergpdA;trpCAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dnase1L3 R206C pIRES-DsRed
Plasmid#98847PurposeExpresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRedDepositorInsertDnase1L3 (DNASE1L3 Human)
UseTagsIRES DsRedExpressionMammalianMutationR206C which greatly reduces nuclease activityPromoterCMVAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-wt
Plasmid#60728PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZS1[FruR-L]
Plasmid#60742PurposeContains PIq driving expression of the FruR-L, the Fructose inducible chimera with the wt Lac DBD.DepositorInsertFruR-L
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with FruR L…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP
Plasmid#190039PurposeExpression of FIP200 mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and MBPExpressionMammalianMutation(641-779)deletion & 1363 RDKDLIES 1370 to GSS…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-L,LacI-L]
Plasmid#60759PurposeContains PIq driving expression GalS-L, and PI driving expression of LacI-L.DepositorInsertsGalS-L
wt LacI
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with GalS L…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pzs1[GalS-L]
Plasmid#60743PurposeContains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.DepositorInsertGalS-L
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with GalS L…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-T]
Plasmid#60751PurposeContains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.DepositorInsertRbsR-T
UseSynthetic BiologyTagsExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-L]
Plasmid#60741PurposeContains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.DepositorInsertRbsR-L
UseSynthetic BiologyTagsExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with RbsR L…PromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
S10A mutant of H3S10ph biosensor
Plasmid#120810PurposeFRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capabilityDepositorInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
UseTagsExpressionMammalianMutationSerine 10 is mutated to Alanine, and Threonine 3,…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1 C122E
Plasmid#232274PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell linesDepositorInsertBACH1 (BACH1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationchanged cysteine 122 to glutamic acidPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only