Addgene

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pFastBacI-KDAC8 H143A

Plasmid

#224344

Purpose

Expresses human KDAC8 (HDAC8) H143A in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 143 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC8 D101E

Plasmid

#224350

Purpose

Expresses human KDAC8 (HDAC8) D101E in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Use

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Aspartate 101 mutated to glutamate

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Use

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pAG668 lifeAct-pFAST

Plasmid

#172866

Purpose

Expresses LifeAct-pFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-pFAST

Use

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac1_GI.1_Q62C-A140C

Plasmid

#162581

Purpose

Expresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Glutamine 62 changed to Cysteine and Alanine 140 …

Promoter

polyhedring

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAG156 Mito-FAST

Plasmid

#130723

Purpose

Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Mito-FAST

Use

Tags

Myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Fast-GCaMP-EF20

Plasmid

#52645

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF20

Use

Tags

Expression

Mammalian

Mutation

D362N/D366N mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastbac-Flag-BRCA1

Plasmid

#223228

Purpose

Insect expression construct for Flag-tagged BRCA1 protein production and purification

Depositor

Weixing Zhao

Article

Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

Insert

Breast Cancer 1 (BRCA1 Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG109 H2B-FAST

Plasmid

#130722

Purpose

Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

H2B-FAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1N-LZ-LOV2(fast)

Plasmid

#107690

Purpose

N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 (MAPRE1 Human)

Use

Tags

A. sativa phototropin 1 LOV2 domain (I427V, H519L…

Expression

Mammalian

Mutation

EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

Promoter

CMV

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Fast-GCaMP-EF16

Plasmid

#52642

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF16

Use

Tags

Expression

Mammalian

Mutation

D431N/D433N/D435N/E442A mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
Fast-GCaMP-EF19

Plasmid

#52644

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF19

Use

Tags

Expression

Mammalian

Mutation

T328D/T330D mutated from GCaMP3

Promoter

T7

Available since

April 23, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac1/MBP-His

Plasmid

#38008

Depositor

Michael J Fox Foundation MJFF

Insert

MBP (Maltose binding protein)

Use

Baculovirus expression

Tags

6xHIS

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits