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Plasmid#63991PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsStrep II (STR)ExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only
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pST50Trc3-STRHISNDHFR
Plasmid#63982PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HPCNDHFR
Plasmid#63980PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-CBPNDHFR
Plasmid#63977PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCalmodulin Binding Peptide (CBP); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-DHFRCYD
Plasmid#63970PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCovalent Yet Dissociable (CYD) [AGTEFCA]ExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGHISNDHFR
Plasmid#63983PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & FLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-STRHISNDHFR
Plasmid#63964PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-CBPHISNDHFR
Plasmid#63963PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Calmodulin Binding Peptide (CBP); TEV…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HPCDHFR
Plasmid#63957PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC)ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCNDHFR
Plasmid#63944PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-DHFRCYD
Plasmid#63952PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsCovalent Yet Dissociable (CYD) [AGTEFCA]ExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HPCDHFR
Plasmid#63939PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsProtein C (HPC)ExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN YFP-DHFR(DD)
Plasmid#29326DepositorInsertYFP-DHFR(DD)
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…PromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
UseTagsDHFR and mNeonExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-mCh-DHFR
Plasmid#173013PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.DepositorInsertTfR-mCh-DHFR
UseTagsDHFR and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN DHFR(DD)-YFP
Plasmid#29325DepositorInsertDHFR(DD)-YFP
UseRetroviralTagsHA and YFPExpressionMutationChange Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …PromoterAvailable sinceJuly 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-PP7-VP64_T2A_GFP
Plasmid#86167PurposeExpresses PP7-VP64 fused to DHFR domain on N-terminus in mammalian cellsDepositorInsertDHFR-PP7-VP64_T2A_GFP
UseCRISPR and LentiviralTagsDestabilized domain (N-terminus version) of E.col…ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLoxP-DHFR-mCherry
Plasmid#70147PurposeExpresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletionDepositorInserta selectable dihydrofolate reductase-thymidylate synthase marker
UseExpression in toxoplasma gondiiTagsmCherryExpressionMutationPromoterDHFR 5' UTRAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only