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pST_pvLEA22mer_mutant_2 (pBS0715)

Plasmid

#185220

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_2. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_17 (pBS0712)

Plasmid

#185219

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_17. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_17

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_16 (pBS0711)

Plasmid

#185218

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_16. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_16

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_14 (pBS0709)

Plasmid

#185217

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_14. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_14

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_pvLEA22mer_mutant_10 (pBS0705)

Plasmid

#185216

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-sg-mGreenLantern332

Plasmid

#179914

Purpose

All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

Depositor

Johan Sosa, Indeever Madireddy

Article

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

mGreenLantern sgRNA

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP-2_6m.mutant

Plasmid

#162842

Purpose

Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

Depositor

Matthias Heinemann

Article

Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

Insert

2_6m_RNA-device

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-Chantico

Plasmid

#170659

Purpose

pUC19 vector containing an insertion of the Chantico promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Chantico

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-Firefly

Plasmid

#163901

Purpose

Luciferase reporter for Chantico lncRNA

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mGreenLantern pDRF1-GW

Plasmid

#168093

Purpose

Expresses mGreenLantern to check for monomericity.

Depositor

Bas Teusink

Insert

mGreenLantern

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIntegrase-AntiDsRed

Plasmid

#139677

Purpose

Cassette for testing phiC31 Integrase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Integrase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pExcisionase-AntiDsRed

Plasmid

#139675

Purpose

Cassette for testing phiC31 Excisionase activity using a fluorescent reporter

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed phiC31 Excisionase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Anti-Sense

Plasmid

#124444

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF8_isoD_CRISPR_resistant

Plasmid

#122468

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform D CRISPR resistant (SCAF8 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Rab28_Anti-Sense

Plasmid

#124442

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

RAB28, member RAS oncogene family (Rab28 Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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Triple mutant

Plasmid

#63719

Purpose

Voltage sensing domain of Ciona with the A154D/R217Q/R229I mutations fused to super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Triple mutant

Use

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA-humanTRAF3 (1098)

Plasmid

#66927

Purpose

Mammalian Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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