Skip to main content
Addgene
Showing: 351 - 400 of 616 results

  1. pGMβ1

    Plasmid
    #216202
    Purpose
    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.
    Depositor
    Yasuko Sasaki
    Article
    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1
    Type
    Empty backbone
    Use
    Shuttle vector, conjugal plasmid, theta type rep…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…
    Available since
    Jan. 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216202-plasmid-map-sequence-id-458484

    Enlarge

  2. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47049-plasmid-map-sequence-id-408250

    Enlarge

  3. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-67070

    Enlarge

  6. YFP-synDAT-S/A

    Plasmid
    #48792
    Purpose
    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro
    Depositor
    Jonathan Javitch
    Article
    Fog et al Neuron. 2006 Aug 17;51(4):417-29.
    Insert
    YFP-synhDAT-S/A (SLC6A3 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…
    Promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    48792-plasmid-map-sequence-id-71096

    Enlarge

  7. YFP-synDAT-S/D

    Plasmid
    #48793
    Purpose
    Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro
    Depositor
    Jonathan Javitch
    Article
    Fog et al Neuron. 2006 Aug 17;51(4):417-29.
    Insert
    YFP-synhDAT-S/D (SLC6A3 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…
    Promoter
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    48793-plasmid-map-sequence-id-71101

    Enlarge

  8. pNUTN6His Y426F/Y517F hTFNG

    Plasmid
    #70084
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70084-plasmid-map-sequence-id-129389

    Enlarge

  33. pRN120

    Plasmid
    #160696
    Purpose
    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator
    Depositor
    Daniel Voytas
    Article
    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103
    Inserts
    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    Use
    Luciferase; Gemini viral replicon, plant developm…
    Tags
    Expression
    Mutation
    Promoter
    AtUbi10, CmYLCV, and Nos
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160696-plasmid-map-sequence-id-316629

    Enlarge

  41. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

    Enlarge

Showing: 351 - 400 of 616 results