-
Plasmid#121027PurposeMoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRiboJ + UTR1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T16m
Plasmid#121035PurposeMoClo golden gate assembly DE part for L3S2P55 (256x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P55 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T17m
Plasmid#121036PurposeMoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S2P11 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T12m
Plasmid#121031PurposeMoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertECK120029600 (strongest natural Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
MZB174
Plasmid#228487Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneUseTags10xHis-SUMO(Smt3)-GST-HRVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB205
Plasmid#228490PurposeEmpty 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneUseTags10xHis-SUMO(Smt3)-Avitag-HRVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
U24m
Plasmid#121026PurposeMoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).DepositorInsertRiboJ + BCD2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T14m
Plasmid#121033PurposeMoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.DepositorInsertL3S1P13 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C90m
Plasmid#120998PurposeMoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.DepositorInsertNahRsfGFP fusion
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C92m
Plasmid#121000PurposeMoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).DepositorInsertsfCFPpdt3 (Chen,Bennett)
UseSynthetic BiologyTagspdt3 mfLon degradation tagExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C99m
Plasmid#121003PurposeMoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).DepositorInsertmScarlet-I codon optimized
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C100m
Plasmid#121004PurposeMoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP with pdt3 tag
UseSynthetic BiologyTagspdt3 mfLon degradation tagExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C101m
Plasmid#121005PurposeMoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).DepositorInsertsfCFP with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C102m
Plasmid#121006PurposeMoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).DepositorInserttreRT with LAA tag
UseSynthetic BiologyTagsLAA degradation tag for ssrAExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C72m
Plasmid#120984PurposeMoClo golden gate assembly CD part for LacI-MVY (Adam Meyer's improved LacI repressor from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertLacI Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C73m
Plasmid#120985PurposeMoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's improved LasR activator, selected for dynamic range and low cross-sensitivity with methods from https://doi.org/10.1101/285866).DepositorInsertLasR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C74m
Plasmid#120986PurposeMoClo golden gate assembly CD part for LuxRQ-FMY (Adam Meyer's improved LuxR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertLuxR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C75m
Plasmid#120987PurposeMoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's improved NahR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertNahR Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P66m
Plasmid#120954PurposeMoClo golden gate assembly AB part for pRpa-AM (Adam Meyer's improved pRPA promoter from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).DepositorInsertpRpa Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICH47732:FCP:ShBle
Plasmid#85985PurposeExpresses ShBle with FCP promoter/terminator for F. cylindrus transformation.DepositorInsertShBle
UseGolden gate assemblyTagsExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP
Plasmid#185678PurposeLow-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.DepositorInsertERmembrane-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TOPO GFP
Plasmid#68488PurposeTOPO construct expressing GFP for generation of GMAP-compatible geneDepositorInsertGreen Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
p006-GFP-Logic-AND
Plasmid#112237PurposeGFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.DepositorInsertsGFP under control of doubly-repressible promoter
GFP
araC
UseTagsExpressionBacterialMutationPromoterAND-gate and pLacIAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b-MBPx-MBPx-SpyTag003
Plasmid#216291PurposeExpresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.DepositorInsertMBPx-MBPx-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R265
Plasmid#138167PurposeExpresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mTagBFP2
Plasmid#68413PurposeTOPO construct expressing mTagBFP2 for generation of GMAP-compatible geneDepositorInsertmTagBFP2
UseGmapTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO CFP
Plasmid#68489PurposeTOPO construct expressing CFP for generation of GMAP-compatible geneDepositorInsertCyan Fluorescent Protein
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO mCherry
Plasmid#68490PurposeTOPO construct expressing mCherry for generation of GMAP-compatible geneDepositorInsertmCherry
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO PuroR
Plasmid#68447PurposeTOPO construct expressing PuroR for generation of GMAP-compatible geneDepositorInsertPuromycin Resistance
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO ZeoR
Plasmid#68446PurposeTOPO construct expressing ZeoR for generation of GMAP-compatible geneDepositorInsertZeocin Resistance
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
TOPO HygroR
Plasmid#68445PurposeTOPO construct expressing HygroR for generation of GMAP-compatible geneDepositorInsertHygromycin Resistance
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FFiBlast p5
Plasmid#68470PurposeInverted and floxed blasticidin resistanceDepositorInsertBlasticidin Resistance
UseCre/LoxTagsExpressionMammalianMutationInvertedPromoterLTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP
Plasmid#185687PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).DepositorInsertERmembrane-GST-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only