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Showing: 281 - 320 of 352 results

  1. pFLIP32

    Plasmid
    #65500
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65500-plasmid-map-sequence-id-111220

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  2. pFLIP39

    Plasmid
    #65507
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65507-plasmid-map-sequence-id-111416

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  3. pFLIP34

    Plasmid
    #65501
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-t7TFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65501-plasmid-map-sequence-id-111221

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  4. pFLIP35

    Plasmid
    #65502
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-TFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65502-plasmid-map-sequence-id-111222

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  5. pFLIP36

    Plasmid
    #65504
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-Cer-cMyc
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65504-plasmid-map-sequence-id-111223

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  6. pFLIP42

    Plasmid
    #65617
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-Cit-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65617-plasmid-map-sequence-id-111419

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  13. pALOD4 non-binding mutant

    Plasmid
    #111027
    Purpose
    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)
    Depositor
    Arun Radhakrishnan
    Article
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A, G501A, T502A, T503A, L5…
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111027-plasmid-map-sequence-id-214042

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  24. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  25. pBB0323-TEV-His12

    Plasmid
    #137039
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66700.1
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137039-plasmid-map-sequence-id-291948

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  26. pBB0238-TEV-His12

    Plasmid
    #137038
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66635.2
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137038-plasmid-map-sequence-id-291947

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  27. pHtrA-TEV-His12

    Plasmid
    #137036
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137036-plasmid-map-sequence-id-275699

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  28. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  29. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  30. pBB0405-TEV-His12

    Plasmid
    #137040
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137040-plasmid-map-sequence-id-275693

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  31. pRevA-TEV-His12

    Plasmid
    #137034
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAF07416.1 (revA Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137034-plasmid-map-sequence-id-275692

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  32. pP13-TEV-His12

    Plasmid
    #137035
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    Use
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137035-plasmid-map-sequence-id-275690

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  33. NLS-tdTomato

    Plasmid
    #163443
    Purpose
    Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone
    Depositor
    Sanford Simon
    Insert
    tdTomato
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163443-plasmid-map-sequence-id-321654

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  34. pGS140_6xHis-EGFP-I3-01

    Plasmid
    #211889
    Purpose
    Bacterial expression of I3-01
    Depositor
    Iain Cheeseman
    Insert
    I3-01
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211889-plasmid-map-sequence-id-426370

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  35. pDK324

    Plasmid
    #134190
    Purpose
    Expression of C. elegans KNL1 with 8A mutation
    Depositor
    Iain Cheeseman
    Insert
    KNL1 (knl-1 Nematode)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    8A
    Promoter
    Available since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    134190-plasmid-map-sequence-id-272307

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  36. HI.HslUV.RSET.FL.wt(V).E258Q(U)

    Plasmid
    #17961
    Depositor
    David McKay
    Insert
    Haemophilus influenzae heat shock loci U and V
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E258Q in hslU gene
    Promoter
    Available since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  38. FN 227

    Plasmid
    #50499
    Depositor
    Kenneth Yamada
    Insert
    FN1 fibronectin 1 (FN1 Human)
    Use
    Tags
    6X-His
    Expression
    Bacterial
    Mutation
    repeats 6-10; D1526E and R1524K
    Promoter
    T7
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 281 - 320 of 352 results