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Plasmid#46266DepositorInsertVcl T12 mutant (VCL Chicken)
UseTagsEGFPExpressionMammalianMutationT12 mutant (D974;K975;R976; R978A) autoinhibition…PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dynamitin-GFP
Plasmid#51419Purposeexpression of GFP-tagged wild-type dynamitin p50 in mammalian cellsDepositorInsertDynamitin (DCTN2 Chicken)
UseTagsGFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I
Plasmid#111827PurposeVinculin tension sensor with point mutation affecting ability to bind talinDepositorInsertVinculinTS (A50I)
UseTagsExpressionMammalianMutationchanged alanine 50 to isoleucinePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
GgVcl 1-1066 FRET tail probe 1
Plasmid#46276DepositorInsertVcl 1-1066 + EYFP (VCL Chicken)
UseTagsECFPExpressionMammalianMutationaddition of 9 bp linker and EYFP at aa 883PromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8s-WPRE
Plasmid#176758PurposeAAV-mediated expression of GCaMP8s under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VinculinI997A_TurboID_Venus
Plasmid#200274PurposeExpressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
UseTagsExpressionMammalianMutationchange isoleucine to alanine at 997 of vinculinPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-deltaOVA
Plasmid#64595PurposeExpression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
UseTagsExpressionMammalianMutationLacking first 49 aa (leader sequence for transloc…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-mDlx-jGCaMP8f-WPRE
Plasmid#176753PurposejGCaMP8f expression in GABA-ergic interneurons under the control of mDlx enhancerDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromotermDlxAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066-ECFP FRET
Plasmid#46179DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP, EYFP, and HisExpressionBacterialMutationaa 884-1066 (Vt, tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-GiP
Plasmid#58540PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTRDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
Core HS4 insulator sequence (dimer)
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-eTLR
Plasmid#214737Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locusDepositorInsertAAVS1-eTLR (PPP1R12C Synthetic, Bovine, CMV, Anguilla japonica, influenza, Rabbit, Chicken, Human)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE
Plasmid#104496PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switchDepositorHas ServiceAAV PHP.eB, AAV Retrograde, and AAV1InsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCAGAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7b-WPRE
Plasmid#104489PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde and AAV1InsertjGCaMP7b
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-CA-Src-GFP
Plasmid#83469PurposeLentiviral vector for doxycycline-inducible expression of constitutively active c-Src in mammalian cellsDepositorInsertSrc (SRC Chicken)
UseLentiviralTagsEGFPExpressionMammalianMutationY527F (constitutively active)Promoterdoxycycline-inducible promoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry-miniSOG-Paxillin-22
Plasmid#57796PurposeLocalization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertPaxillin
UseTagsPA-mCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7c variant 1513-WPRE
Plasmid#105321PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoterDepositorHas ServiceAAV1InsertjGCaMP7c variant 1513
UseAAVTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV::TVA/tRFP
Plasmid#164486PurposeDual CMV promoter plasmid to express TVA and TurboRFPDepositorInsertsUseTagsExpressionMammalianMutationPromotersCMV and sCMV IE94Available sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
Plasmid#68719PurposeCre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.DepositorHas ServiceAAV1InsertmRuby2-P2A-GCaMP6f
UseAAVTagsExpressionMutationPromoterCAG-FLEXAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK284 (Stag-3FLAG-Hygro)
Plasmid#72800PurposeC-terminal tagging with Stag-3FLAG using CRIPSR/Cas9DepositorInsertStag-3FLAG-Hygro
UseCRISPRTagsStag-3FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK278 (mClover-Hygro)
Plasmid#72794PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Hygro
UseCRISPRTagsmCloverExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only