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Plasmid#164959PurposeZebrafish kcnn3 gene CDSDepositorInsertkcnn3 (kcnn3 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK004 ubi:mCerulean_SV40 (#302)
Plasmid#195978PurposeExpression constructDepositorInsertubi:mCerulean
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E Lck
Plasmid#135201PurposeT-cell markerDepositorInsertLck promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
flk1 promoter
Plasmid#26436DepositorInsertflk1 promoter
UseTopo cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA
Plasmid#105648PurposeExpression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeksDepositorInsertTol2-Isl2b:GFP:polyA (isl2b Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn4
Plasmid#164960PurposeZebrafish kcnn4 gene CDSDepositorInsertkcnn4 (kcnn4 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ Zf ctnna1L344P
Plasmid#185407PurposeZebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMVDepositorInsertctnna1 (ctnna1 Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Leucine 344 to ProlinePromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-mRFP
Plasmid#117772PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7 (hspb7 Zebrafish)
UseTagsHalo-TEVExpressionMammalianMutationPromoterSP6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E_he1a:ECFP
Plasmid#113880Purposep3E entry vector for Three Multisite GatewayDepositorInserthe1a (he1.1 Zebrafish)
UseZebrafish danio expressionTagsECFPExpressionMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZE33 Prickle-1-GFPnopren-pCS2+
Plasmid#17074DepositorInsertPrickle-1 (prickle1a Zebrafish)
UseZebrafish expressionTagsGFPExpressionMutationPk1GFPnopren deletes the C-terminal six amino aci…PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
UseTagsEGFPExpressionMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
acta2_mCherry_pA_pDestTolpA2
Plasmid#78685Purposeacta2:mCherry transgenesis constructDepositorInsertacta2 promoter (acta2 Zebrafish)
UseUnspecifiedTagsmCherryExpressionMutationPromoteracta2Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneTagsExpressionMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP
Plasmid#158963PurposeCre-dependet Cas9-GFP expressionDepositorInsertshsp70l promoter
floxed Non-FP cassette
Cas9-GFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorInsertdopamine transporter (slc6a3 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterdatAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only