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pLenti_EF1a_Flag_TP53_R248W+P278A

Plasmid

#183115

Purpose

Expresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed proline 278 to alanine and arginine 248 t…

Promoter

EF1a

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT1_p.Y701F

Plasmid

#82183

Purpose

Gateway Donor vector containing STAT1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT1 (STAT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y701F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FGFR3_WT

Plasmid

#82121

Purpose

Gateway Donor vector containing FGFR3 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FGFR3 (FGFR3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERRFI1_WT

Plasmid

#82116

Purpose

Gateway Donor vector containing ERRFI1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERRFI1 (ERRFI1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CCNG1_WT

Plasmid

#81767

Purpose

Gateway Donor vector containing CCNG1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CCNG1 (CCNG1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-EGFP-GPI-stop-L2

Plasmid

#186355

Purpose

Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

signal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Synthetic, Human)

Use

Expression of a fluorescent membrane marker

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61K

Plasmid

#81319

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Q61K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K1_p.S218D_S222D

Plasmid

#82177

Purpose

Gateway Donor vector containing MAP2K1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S218D, S222D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR1A_Mavs (open)

Plasmid

#160099

Purpose

Expresses murine Mavs in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Mavs (Mavs Mouse)

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_GEN1_WT

Plasmid

#81844

Purpose

Gateway Donor vector containing GEN1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GEN1 (GEN1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S92T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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