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Plasmid#162512PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoterDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterhSYNAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorTagsExpressionMutationPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMALB
Plasmid#161784PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorTagsExpressionYeastMutationPromotergapdh, pyk1, and tef1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL
Plasmid#161783PurposeGateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and hPGKAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRai
Plasmid#189938PurposeExpresses CRISPRai cassette under EF1a promoterDepositorInsertCRISPRai cassette
UseTagsExpressionMammalianMutationPromoterCMV enhancer-rat EF1 alphaAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1-FL- luciferase
Plasmid#135566PurposeFull Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferaseDepositorInsertFull-length promoter of human retinoic acid receptor-beta gene
UseTagsExpressionMutationPromoterhHuman CYP26A1 gene promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)
Plasmid#110560PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insertDepositorInsertstRBS-lacZ
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRB17
Plasmid#52527Purposeserves as template for PCR-mediated fusion of U6-promoter with sgRNADepositorInsertDrosophila U6C promoter
UseCRISPR; Blunt cloning kitTagsT7-promoterExpressionInsectMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hST3GAL6-RFP
Plasmid#22923DepositorInserthuman type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
SGL40C-H1.EFS.RFP657
Plasmid#69148PurposeAdvanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1P-E4-luciferase
Plasmid#135592PurposeShort form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vectorDepositorInsertShort form promoter of human CYP26A1 gene (E4)
UseTagsExpressionMutationPromoterHuman CYP26A1 promoter (Short Form, E4)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-GFP-WPRE
Plasmid#44352DepositorInsertEF1a-GFP
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2526-3969)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
NSSCP
Plasmid#183190PurposemRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEVDepositorInsertsmRFP-PopZ
DivIVA-GFP(tevR-50)
SpmX-TEV(C)
TEV(N)-SpmX
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110561PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertstRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
SGL40C.EFS.RFP657
Plasmid#69147PurposeAdvanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)DepositorInsertssgRNA
EFS
tagRFP657
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
ShuttleMCP
Plasmid#50959PurposeA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoterDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoteruses portion of the mouse cofilin gene as a mouse…Available sinceFeb. 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits