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Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+HIF1a-AS1_deltaExon1 (nucleotides 26-78)
Plasmid#194175PurposeExpresses lncRNA HIF1a-AS1 mutantDepositorInsertHIF1a-AS1 (HIF1A-AS1 Human)
UseTagsExpressionMammalianMutationWithin Exon1, nucleotides 26-78 were removedPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tau Authentic Alu 10-12WT
Plasmid#194167PurposeInsert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.DepositorInsertMicrotubule-associated protein tau 10-12 WT
UseTags3X FlagExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tau Authentic Alu 7-12WT
Plasmid#194170PurposeInsert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.DepositorInsertmicrotubule-associated protein tau 7-12 WT
UseTags3x FlagExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tau Authentic Alu 10-12K317M
Plasmid#194168PurposeInsert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.DepositorInsertMicrotubule-associated protein tau 10-12 K317M
UseTags3X FlagExpressionMammalianMutationChanged Lysine 317 to MethioninePromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SOD1 C57D/C146D
Plasmid#191848Purposeexpress C57D/C146D mutant SOD1 in mammalian cellsDepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationC57D, C146DPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4_377-428_AG
Plasmid#148757PurposeMammalian Expression of HsNot4_377-428DepositorInsertHsNot4_377-428 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472_AC
Plasmid#148419PurposeMammalian Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1B-FUS-IDR
Plasmid#183240PurposeExpression of HNF1B fused to a intrinsically disordered regions of the FUS proteinDepositorInsertHNF1 homeobox B (HNF1B Human)
UseTagsFusion to an IDR from the FUS protein and FLAG ta…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1A-delta-IDR2
Plasmid#183238PurposeExpression of FLAG tagged HNF1A with deletion of a intrinsically disordered regions (AA545-573)DepositorInsertHNF1 homeobox A (HNF1A Human)
UseTagsFLAG tagExpressionMammalianMutationDeletion of amino acids 545-573PromoterCMVAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) HuTF-Fc-His tag
Plasmid#183253PurposeSoluble expression of the extracellular domains of human tissue factor (TF)-FcDepositorInsertTissue factor (F3 Human)
UseTags8xHisExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat(G1A)-mCherry
Plasmid#181852PurposemCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationGlycine residue immediately after start site in P…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I)
Plasmid#181861PurposeFluoSTEP-AKAR using mScarlet-I as the FRET acceptor.DepositorInsertFluoSTEP-AKAR (mScarlet-I)
UseTagsmScarlet-I and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H
Plasmid#177767PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationR9APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H
Plasmid#177768PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationS95APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H
Plasmid#177769PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationK116APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H
Plasmid#177770PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationT139APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H
Plasmid#177771PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationK370APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H
Plasmid#177772PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationS374APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H
Plasmid#177773PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)DepositorInsertGRHL1 (GRHL1 Human)
UseTagsExpressionMammalianMutationK592APromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only