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Plasmid#104565PurposeMammalian expression vector for DmrC fused to VPR activatorDepositorInsertDmrC-VPR
UseTags3x FLAG and NLSExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter
Plasmid#65007PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning siteDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…PromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR
Plasmid#118468Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertsapphireCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR
Plasmid#118472PurposecpBFP-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertblueCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5xE-Box
Plasmid#118052PurposeGBPart - Operator/DNA Response element - 5 copies of the E-box motifDepositorInsert5 copies of the E-box motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt(R23/25A)
Plasmid#181835PurposePhospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.DepositorInsertLyn-dInPAkt
UseTagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationArginines 23 and 25 in PH domain mutated to alani…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
STAgR_tdTomato
Plasmid#102993PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsTagsExpressionMutationPromoterU6Available sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11299: pHR-HSP-ABE8e-hyperdCas12a-EPS-puro
Plasmid#210605Purposeheat shock promoter - activated hyperdCas12a-based transcriptional base editorDepositorInsertABE8e-hyperdCas12a
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCdkn2a/Cre
Plasmid#89644PurposeExpresses a Cdkn2a-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Cdkn2a (Cdkn2a Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-PlcR
Plasmid#181830PurposeGenetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.DepositorInsertLyn-PlcR
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ144-ZmUbi-tRNA
Plasmid#158402PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-BFP
Plasmid#153426PurposeRetroviral (MSCV) expression of mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-sgKeap1/Cre
Plasmid#89645PurposeExpresses a Keap1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Keap1 (Keap1 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP
Plasmid#169328PurposeExpression of HaloTag9 located at the microtubules of mammalian cellsDepositorInsertCEP41-HaloTag9 (CEP41 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_mU6
Plasmid#102844PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only