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MXS_rtTA3

Plasmid

#62428

Purpose

MXS_chaining vector with rtTA3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Reverse tetracycline transactivator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pJC175e-DddI

Plasmid

#179693

Purpose

Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBCL2-FL(pA)

Plasmid

#42594

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

BCL2 5'UTR - firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEPYCeGM0001

Plasmid

#196141

Purpose

Gateway entry vector with Arabidopsis TGA1 CDS and c-terminal HiBiT tag

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

AtTGA1_HiBit

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN-ΔC

Plasmid

#32794

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 267-522

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
Trip-GFP-hOCLN-ΔEL2

Plasmid

#32797

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of aa 200-238

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER shLUC (w177-1)

Plasmid

#17472

Purpose

Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pAG330 td-FAST

Plasmid

#130810

Purpose

Expresses td-FAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

td-FAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-C (w324-1)

Plasmid

#17404

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame C

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pME-BASU-NS

Plasmid

#166565

Purpose

Multisite Gateway middle entry clone encoding BASU. Parton lab clone JRO

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

BASU

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL7004

Plasmid

#136943

Purpose

Malachite green aptamer, T7 promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

malachite green aptamer (MGA)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pT7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-B (w323-1)

Plasmid

#17403

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame B

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MXS_PGK

Plasmid

#62414

Purpose

MXS_chaining vector with PGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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phpBCL2-FL(pA)

Plasmid

#42593

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Trip-GFP-hOCLN-ΔN

Plasmid

#32792

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-63

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4-FKBP12DD N-term B (w385-4)

Plasmid

#17415

Purpose

Gateway entry vector for Degron, N-term, frame B

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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