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Showing: 1541 - 1560 of 7894 results
  1. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pCRISPR3_BC6

    Plasmid
    #191861
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pCRISPR3_BC5

    Plasmid
    #191860
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pCRISPR3_BC4

    Plasmid
    #191859
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pCRISPR3_BC3

    Plasmid
    #191858
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pCRISPR3_BC1

    Plasmid
    #191856
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pLY86

    Plasmid
    #130949
    Purpose
    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
    Depositor
    Insert
    sgRNA-LEA2-WTmis2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plux2
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pEPOR2KN0076

    Plasmid
    #117643
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)
    Depositor
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  14. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1541 - 1560 of 7894 results