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Showing: 1541 - 1560 of 7972 results

  1. LentiGuide-Hygro

    Plasmid
    #139462
    Purpose
    Lentiviral vector with gRNA scaffold and hygromycin selectable marker
    Depositor
    Caroline Goujon
    Insert
    no sgRNA inserted; resistance gene: hygroR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139462-plasmid-map-sequence-id-308474

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  2. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  3. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Expression
    Mutation
    Promoter
    EFS (P2A)
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187646-plasmid-map-sequence-id-367454

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  4. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  9. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191857-plasmid-map-sequence-id-381604

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  10. pCRISPR3_BC6

    Plasmid
    #191861
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191861-plasmid-map-sequence-id-379165

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  11. pCRISPR3_BC5

    Plasmid
    #191860
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191860-plasmid-map-sequence-id-379164

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  12. pCRISPR3_BC4

    Plasmid
    #191859
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191859-plasmid-map-sequence-id-379162

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  13. pCRISPR3_BC3

    Plasmid
    #191858
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191858-plasmid-map-sequence-id-379160

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  14. pCRISPR3_BC1

    Plasmid
    #191856
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191856-plasmid-map-sequence-id-379154

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  15. pLY86

    Plasmid
    #130949
    Purpose
    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEA2-WTmis2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plux2
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    130949-plasmid-map-sequence-id-258930

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  16. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    117645-plasmid-map-sequence-id-240245

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  17. pEPOR2KN0076

    Plasmid
    #117643
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    117643-plasmid-map-sequence-id-228395

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  18. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  19. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  20. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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Showing: 1541 - 1560 of 7972 results