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Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+(rInsp)-Ins-GLuc
Plasmid#89928PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoterrat Insulin II promoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGV13 (CK2alpha K68M, CK2beta)
Plasmid#27094DepositorUseTetracycline-regulated expressionTagsHA on CK2alpha and Myc on CK2betaExpressionMammalianMutationK68M on CK2alphaPromoterbidirectional tet-responsive promoter and bidirec…Available sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.5'.GFP
Plasmid#59407Purposeexpresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsertapprox. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterFOXA2Available sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGfa2(ABDx3)-nLac
Plasmid#53133Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of 3 copies of the gf…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa(B3)-nLac
Plasmid#53132Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of the gfa2 B segment…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb
Plasmid#183080PurposerAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateTagsExpressionMutation-124C>T promoter, Exon 1 C7C (C21>T21) sile…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb
Plasmid#183081PurposerAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateTagsExpressionMutation-146C>T promoter, Exon 1 C7C (C21>T21) sile…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_TERT_prom_C7C_1.8kb
Plasmid#183079PurposerAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateTagsExpressionMutationWildtype promoter, Exon 1 C7C (C21>T21) silent…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ADP-MCS-FLAG
Plasmid#192360PurposeAdipocyte-specific overexpression of genesDepositorTypeEmpty backboneUseAAVTags3xflagExpressionMammalianMutationPromoteradiponectin promoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-Cre-blast
Plasmid#179390PurposeLentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.DepositorInsertmCherry (P2A) Cre recombinase
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) WT
Plasmid#45383DepositorInsert6 copies of hPAI-1 promoter -732/-721
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
C3 luc (T1 del)
Plasmid#11358DepositorInsertC3 promoter (C3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN
Plasmid#66787PurposeExpression of murine PDGFRalpha tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-eGFP
Plasmid#193022PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the cytomegalovirus (CMV) promoterDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Fosprom
Plasmid#188113PurposeExpresses luciferase under control of Fos promoter in transfected cellsDepositorInsertFos promoter (Fos Mouse)
UseLuciferaseTagsExpressionMutationPromoterFos endogenous promoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPP6
Plasmid#209968PurposeContains Level 0 Part: Promoter Dropout (sfGFP) for the construction of Level 1 plasmids and subsequent promoter insertionDepositorInsertPromoter Dropout (sfGFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMAL
Plasmid#161785PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and GFP.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sf-U7snRNA-ESE-AS-NsiI
Plasmid#190694PurposeBasic vector used for the expression of U7-based antisense oligonucleotidesDepositorInsertU7 snRNA
UseLentiviralTagsExpressionMammalianMutationPromoterU7 promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSMALB
Plasmid#161786PurposeGateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.DepositorTypeEmpty backboneUseLentiviralTagsTagBFPExpressionMammalianMutationPromoterBidirectional minhCMV and SFFVAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only