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Addgene
Showing: 1541 - 1560 of 3953 results

  1. pNK845

    Plasmid
    #219746
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal luciferase
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mutation
    I3S, N4T, F11L, I63T, T99P, T192S, A199P
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    219746-plasmid-map-sequence-id-437111

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  5. pIS0

    Plasmid
    #12178
    Purpose
    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production
    Depositor
    David Bartel
    Article
    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    12178-plasmid-map-sequence-id-181181

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  6. psicheck_NDUFA6_3UTR_mut

    Plasmid
    #65971
    Purpose
    Luciferase assay vector, assessing regulation of NDUFA6 3' UTR, with two mutated tandem CAC motifs
    Depositor
    Thomas Tuschl
    Insert
    NDUFA6 (NDUFA6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated tandem CAC motif to CCC motif
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  8. pGL3-pSMANTIS_1kb_1 KLF2mut 449,899

    Plasmid
    #194198
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Availability
    Academic Institutions and Nonprofits only
    194198-plasmid-map-sequence-id-381969

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  9. pGL3-pSMANTIS_1kb_1 KLF2mut 449,548

    Plasmid
    #194199
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,548)
    Promoter
    SMANTIS promoter (1kb), mutated
    Availability
    Academic Institutions and Nonprofits only
    194199-plasmid-map-sequence-id-381923

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  10. pGL3-pSMANTIS_1kb_1 KLF2mut 449,732

    Plasmid
    #194200
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,732)
    Promoter
    SMANTIS promoter (1kb), mutated
    Availability
    Academic Institutions and Nonprofits only
    194200-plasmid-map-sequence-id-381961

    Enlarge

  11. pGL3-pSMANTIS_1kb_1 KLF2mut 449,884

    Plasmid
    #194201
    Purpose
    Includes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    2 mutated KLF binding sites (position 449,884)
    Promoter
    SMANTIS promoter (1kb), mutated
    Availability
    Academic Institutions and Nonprofits only
    194201-plasmid-map-sequence-id-381985

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  12. pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT

    Plasmid
    #212182
    Purpose
    This binary vector expresses Nanoluciferase (Nluc) containing the Argonaut (AGO) intron (405 bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    NanoLuc luciferase-I{AGO-405}
    Use
    Tags
    Expression
    Plant
    Mutation
    AGO intron added at bp 405, eliminates expression…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
    212182-plasmid-map-sequence-id-422934

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  13. pLSU1/t35s[]:nnLuz-I{PIV-216}:NosT

    Plasmid
    #212187
    Purpose
    This binary vector expresses mushroom luciferase (nnLuz) containing the PIV intron (216 bp into the gene) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    nnLuz-I{PIV-216}
    Use
    Tags
    Expression
    Plant
    Mutation
    PIV intron added at bp 216, eliminates expression…
    Promoter
    truncated 35sCaMV
    Availability
    Academic Institutions and Nonprofits only
    212187-plasmid-map-sequence-id-422962

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  16. pGL3 basic E2

    Plasmid
    #48747
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. pGL3 Pro E2

    Plasmid
    #48749
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1541 - 1560 of 3953 results