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pcdna3 Flag Jip1b

Plasmid

#52123

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-JNKKTRClover

Plasmid

#59139

Purpose

ORF encoding JNK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 beta2

Plasmid

#20355

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta2 (MAPK11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4

Plasmid

#14615

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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SuperClomeleon

Plasmid

#203724

Purpose

An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

Depositor

George Augustine

Article

Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

Insert

Cerulean linked topaz fluorescent protein

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Cerulean, modified Topaz Fluorescent Protein with…

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6-AMPK alpha1 (1-312)

Plasmid

#162131

Purpose

Expression vector for constitutively active AMPK alpha 1

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2016 Apr;18(4):431-42. do

Insert

AMPK alpha1 (1-312) (PRKAA1 Rat)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3C

Plasmid

#137758

Purpose

Expression of LC3C

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-mPKR-FLAG-IG

Plasmid

#101792

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse PKR

Depositor

Seiichi Oyadomari

Article

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Pkr (Eif2ak2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(E17K)

Plasmid

#86563

Purpose

Expresses human full-length Akt1(E17K) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

E17K mutation in the PH domain

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRc/RSV Flag MKK3(glu)

Plasmid

#14670

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK3(glu) (MAP2K3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-189Glu Thr-193Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
903 pLNCX HA Akt1

Plasmid

#9004

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
896 pcDNA3 T7 Akt1

Plasmid

#9003

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Use

Tags

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-parkin W403A

Plasmid

#45876

Depositor

Edward Fon

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

W403A mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2a2(apf)

Plasmid

#13761

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2(apf) (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif THr(183)-Pro-T…

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(glu)

Plasmid

#14813

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(glu) (MAP2K4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Glu Thr-224Glu

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only