Addgene

We narrowed to 6,703 results for: 11

Showing: 1541 - 1560 of 6703 results

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pICH51288

Plasmid

#50269

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-NFIA1.1

Plasmid

#112698

Purpose

Expression of NFIA1.1 protein for transient transfection assays

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

Insert

Nuclear Factor I A1.1 (Nfia Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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UnReg-H2B-YFP-DD

Plasmid

#96892

Purpose

Reporter to monitor translation of unstructured mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

UnReg-H2B-Venus-DD

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA6

Plasmid

#26682

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 6 (KPNA6 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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EcMBP165-cpGFP.PPYF.T203V.pRSET

Plasmid

#33373

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

Improved MBP based Maltose Biosensor

Use

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV-40

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
Actinin-sstFRET-GR

Plasmid

#83416

Purpose

FRET-based actinin tension sensor

Depositor

Tetsuya Kitaguchi

Article

Wang et al J Biomech Sci Eng (2016). Vol.11, No.4

Insert

Actinin-sstFRET-GR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only
pLoxP-DHFR-mCherry

Plasmid

#70147

Purpose

Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

Depositor

David Sibley

Article

Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

Insert

a selectable dihydrofolate reductase-thymidylate synthase marker

Use

Expression in toxoplasma gondii

Tags

mCherry

Expression

Mutation

Promoter

DHFR 5' UTR

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-FAM134Bs

Plasmid

#128261

Purpose

Expresses FAM134Bs in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134Bs (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-RTN3L

Plasmid

#128264

Purpose

Expresses RTN3L in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

RTN3 (RTN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-TMEM192-mGFP

Plasmid

#184897

Purpose

Stable expression of TMEM192-mGFP in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

TMEM192 (TMEM192 Human)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101104

Purpose

Encodes dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-mUbp43

Plasmid

#12454

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (Usp18 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pC0091

Plasmid

#181955

Purpose

Beta catenin reporter control M51 Super 8x (mutated TCF/LEF binding sites) FOPFlash with Gluc/Cluc

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

7xFOP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3R1

Plasmid

#174191

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Gq sensor

Plasmid

#137810

Purpose

A FRET sensor for Gq activation, encoded on a single plasmid

Depositor

Dorus Gadella

Article

Goedhart et al PLoS One. 2011;6(11):e27321. doi: 10.137

Insert

Gq sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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