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Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR2b-mTurquoise2
Plasmid#191014PurposeGateway entry vector with the mTurquoise2 fluorescent proteinDepositorInsertmTurquoise2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cdt1-mCherry-Puro
Plasmid#193763PurposeDoxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertCdt1-mCherry (CDT1 Human)
UseLentiviralTagsWTExpressionMutationhuman Cdt1 amino acids 1-100 w/ ĪCy mutation (aa6ā¦PromoterTREAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-TTLL11
Plasmid#140582PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA TTLL11
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
human ETV6 gRNA-1
Plasmid#133353Purposehuman ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.DepositorInsertETV6 (ETV6 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)
Plasmid#120297PurposeAAV vector for expression of AcrIIA4 (no miR binding sites, control vector)DepositorInsertAcrIIA4
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV CMV-driven AcrIIA4-2xmiR-122 target sites
Plasmid#120298PurposeAAV vector for expression of AcrIIA4 with two miR-122 binding sitesDepositorInsertAcrIIA4-2xmiR-122 binding sites
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.10_Gag-MCP-P2A-eGFP
Plasmid#205525PurposeExpress HIV Gag-MCP-P2A-eGFPDepositorInsertGag-MCP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPSU150
Plasmid#173836PurposeExpresses 150 kD Penn State ladder proteinDepositorInsertSTRHSTPABMBPpepN
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
gag-PE
Plasmid#211371PurposePackaging plasmid for v3 PE-eVLP productionDepositorInsertMMLVgag-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP2K2 gRNA (BRDN0001147718)
Plasmid#77601Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K2DepositorInsertMAP2K2 (MAP2K2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC67907
Plasmid#211794PurposeOver expresses Cas9 and GR-GRF-GIF. Must receive guide cassettesDepositorInsertHptII, Cas9, GR-GRF-GIF
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAP2K2 gRNA (BRDN0001147931)
Plasmid#77603Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K2DepositorInsertMAP2K2 (MAP2K2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG1159 TOM20-ECFP-FIREmate
Plasmid#196693PurposeExpresses TOM20-ECFP-FIREmate in mammalian cellsDepositorInsertTOM20-ECFP-FIREmate
UseTagsFIREmateExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4/Full length FVIII
Plasmid#41036DepositorInsertFVIII (F8 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
UseTagsGCaMP 6s RS1 EF4ExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Cas9-EGFP
Plasmid#159126PurposeExpresses active Cas9 fused with EGFP for fluorescence microscopyDepositorInsertCas9 fused to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_FLUCV_HE
Plasmid#207275PurposeMammalian expression of Influenza C virus HE proteinDepositorInsertFLUCV_HE
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-shRNA-Control
Plasmid#98398PurposeLentivirus for expression of non-targeting control shRNA (Dox-inducible)DepositorInsertnon-targeting randomized sequence
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA
Plasmid#128034PurposeMammalian expression vector with N terminal HA tagDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-EGFP (BPK1098)
Plasmid#133962PurposeCMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcriptionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ai9
Plasmid#22799DepositorInsertCAG-Floxed tdTomato
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCALM1-EYFP-WPRE-bGHpA
Plasmid#213971PurposeAAV expression of EYFP from pCALM1 promoterDepositorInsertpCALM1
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (fl)
Plasmid#137820Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v6-10 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull lengthPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
UseTagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mCherry
Plasmid#50462PurposeDouble floxed mCherry under the control of EF1a promoterDepositorInsertmCherry
UseAAVTagsN/AExpressionMutationPromoterEF1aAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-VSVgSS-SNAP-TM
Plasmid#207319PurposeFunctional incorporation of SNAP-tag into envelope of virionsDepositorInsertSNAP-tag
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-ATF4 mScarlet NLS
Plasmid#115969PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-IDepositorInsertactivating transcription factor 4 (ATF4 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorInsertAlpha-Synuclein-His (SNCA Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a geneā¦ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only