Addgene

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Showing: 1521 - 1540 of 9036 results

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PsaA

Plasmid

#159997

Purpose

Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

PsaA (19-309)

Use

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-C-GFP

Plasmid

#172973

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet-STOP

Plasmid

#140876

Purpose

Multisite Gateway middle entry vector for expression of untagged mScarlet.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mScarlet

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pABY-c25

Plasmid

#201769

Purpose

Expresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Use

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pABY-c15

Plasmid

#201768

Purpose

Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

Depositor

Saravanan Palani

Article

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

NbALFA

Use

Tags

40aa Linker, mNeonGreen

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-2-Ubc9 (w345-2)

Plasmid

#17407

Purpose

Gateway entry vector for N-term V5 tag, frame B and Ubc9 C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

Ubc9 and V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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EC59103

Plasmid

#191785

Purpose

LbCas12a expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas12a+ 8 introns-T-E9

Use

CRISPR

Tags

Expression

Plant

Mutation

D156R

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS5x

Plasmid

#155113

Purpose

5' entry vector containing QUAS5x promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Use

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pJB01

Plasmid

#111957

Purpose

Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

RFP

Use

Tags

HaloTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Article

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pJK240

Plasmid

#71127

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

GyrA intein and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-LssmOrange

Plasmid

#96937

Purpose

Retroviral plasmid containing LssmOrange for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Long Stokes Shift Monomeric Orange

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4a G12V

Plasmid

#83169

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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ss-cfSGFP2

Plasmid

#37535

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

ss-cfSGFP2 (cysteine-free SGFP2)

Use

Tags

signal sequence of α1-antitrypsin (M1-K34)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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