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Addgene
Showing: 1521 - 1540 of 4790 results
  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1_Tau Authentic Alu 10-12WT

    Plasmid
    #194167
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Insert
    Microtubule-associated protein tau 10-12 WT
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1_Tau Authentic Alu 10-12K317M

    Plasmid
    #194168
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau K317M mutant. Expresses tau circular RNA 12-->10 KM with 3X flag tag in exon 10.
    Depositor
    Insert
    Microtubule-associated protein tau 10-12 K317M
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Changed Lysine 317 to Methionine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid
    #194169
    Purpose
    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.
    Depositor
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Use
    Tags
    3X flag
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1_Tau Authentic Alu 7-12WT

    Plasmid
    #194170
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Insert
    microtubule-associated protein tau 7-12 WT
    Use
    Tags
    3x Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1521 - 1540 of 4790 results