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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Evelina Tutucci, Robert Singer

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Use

Tags

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Promoter

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002-MBP

Plasmid

#102831

Purpose

Bacterial expression of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to the N-terminus of MBP via a …

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-TEV

Plasmid

#69929

Purpose

bacterial expression of MBP-TEV n-terminal fusions

Depositor

Zita Balklava, Thomas Wassmer

Article

Currinn et al Cell Mol Life Sci. 2015 Jul 28.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

MBP-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a hMDH1v3 (+TEV)

Plasmid

#204229

Purpose

Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

hMDH1v3 (MDH1 Human)

Use

Tags

6X His

Expression

Bacterial

Mutation

Inserted a G after ATG to maintain kozak site

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Bst LF-6xHis

Plasmid

#159148

Purpose

expresses His-tagged large fragment of Bst polymerase

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Insert

large fragment of Geobacillus stearothermophilus DNA polymerase I

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Bxb1-His

Plasmid

#123132

Purpose

Bacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Bxb1 integrase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK522-FAST-PETase

Plasmid

#214754

Purpose

plasmid for expression FAST-PETase in Pseudomonas putida

Depositor

Hal Alper

Article

Lu et al Nature. 2022 Apr;604(7907):662-667. doi:

Insert

FAST-PETase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pETcon_SARS-CoV-2_RBD

Plasmid

#166782

Purpose

Yeast surface display vector, expressing the SARS-2 RBD, 201aa construct

Depositor

Jesse Bloom

Article

Starr et al Cell. 2020 Sep 3;182(5):1295-1310.e20. d

Insert

Spike receptor binding domain (RBD) from SARS-CoV-2 (S )

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET21TtTERT(195-516)

Plasmid

#28172

Depositor

Kathleen Collins

Article

O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

Insert

Tetrahymena TERT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET/ELP-I-CAT

Plasmid

#71461

Purpose

ELP-intein purification system

Depositor

David Wood

Article

Banki et al Nat Methods. 2005 Sep;2(9):659-61.

Type

Empty backbone

Use

Tags

ELP ((VPGXG)110) and intein

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a-hPKM1

Plasmid

#44241

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M1 (PKM Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher-mi3

Plasmid

#112255

Purpose

Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

Insert

SpyCatcher-mi3

Use

Tags

C-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Use

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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