Ypd1p-pET22b
(Plasmid
#12655)
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET22b(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5493
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYPD1 protein
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1003
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GenBank IDZ74283
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Entrez GeneYPD1 (a.k.a. YDL235C)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ypd1p-pET22b was a gift from SeWon Suh (Addgene plasmid # 12655 ; http://n2t.net/addgene:12655 ; RRID:Addgene_12655) -
For your References section:
Insights into eukaryotic multistep phosphorelay signal transduction revealed by the crystal structure of Ypd1p from Saccharomyces cerevisiae. Song HK, Lee JY, Lee MG, Moon J, Min K, Yang JK, Suh SW. J Mol Biol. 1999 Nov 5. 293(4):753-61. 10.1006/jmbi.1999.3215 PubMed 10543964