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pCMV5B-TFGbeta receptor II K277R

Plasmid

#11762

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Use

Tags

His

Expression

Mammalian

Mutation

K277R: kinase dead

Promoter

CMV

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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PCMV-intron myc Rab11WT

Plasmid

#46785

Purpose

expression of WT Rab11a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB11A (RAB11A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ (∂WW)

Plasmid

#24811

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 111-158.

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCMV5-Flag-mEPLIN alpha

Plasmid

#40955

Depositor

Elizabeth Luna, Scott Maul

Article

Maul et al Gene. 2001 Jan 10;262(1-2):155-60.

Insert

EPLIN alpha (Lima1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-CRY2(D387A)-mCherry

Plasmid

#58369

Purpose

Light insensitive CRY2 mutant

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Insert

CRY2[1-498] (D387A) (CRY2 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D387A

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam DP1

Plasmid

#37968

Depositor

Jacqueline Lees

Article

Moberg et al Mol Cell Biol. 1996 Apr;16(4):1436-49.

Insert

DP1 (TFDP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S109A

Plasmid

#33088

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

S109A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
Myc-Foxo1 AAA (pCMV5)

Plasmid

#17547

Depositor

Domenico Accili

Insert

Foxo1 AAA (Foxo1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Changed T24A T253A S316A

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-LOx-IRES-EGFP

Plasmid

#166877

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y132D

Plasmid

#113425

Purpose

Expresses hA3A-BE3-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-CRY2PHR-TevC

Plasmid

#89877

Purpose

expresses NES-CRY2PHR-TevC of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

NES-CRY2PHR-TevC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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Nxn-1 beta Flag/pCMV

Plasmid

#197339

Purpose

Plasmid expressing an antigen targeting Neurexin 1beta

Depositor

Thomas Südhof

Insert

Neurexin 1beta (Nrxn1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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1247 pCMVb p300 HA delta33

Plasmid

#10719

Depositor

William Sellers

Insert

p300 delta33 (EP300 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Mutant does not bind to E1A. Amino acids 1737 to…

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-luc-miR-122

Plasmid

#171557

Purpose

a positive control reporter with 3' UTR sequences perfectly complementary to miR-122

Depositor

Yan Zeng

Article

Li et al Cells. 2019 Jul 30;8(8). pii: cells80807

Insert

a sequences perfectly complementary to miR-122

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Use

Tags

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV Flag cycT2b (P#1718)

Plasmid

#14599

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

A137T compared to NP_490595.1

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
M69 pCMV pGL3 Luciferase

Plasmid

#17186

Depositor

Randall Moon

Type

Empty backbone

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Article

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5B-TGFbeta receptor II wt (N-term HA)

Plasmid

#24801

Depositor

Jeff Wrana

Insert

TGFbeta receptor II (TGFBR2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits