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pTA-Luc SIRT1 promoter (-202)

Plasmid

#10971

Depositor

Toren Finkel

Article

Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

Insert

SIRT1 promoter (Sirt1 Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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EcSTH-LbNOX

Plasmid

#211924

Purpose

This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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PREX2 pcdna3.1 V5/his

Plasmid

#41555

Depositor

Ramon Parsons

Article

Fine et al Science. 2009 Sep 4;325(5945):1261-5.

Insert

PREX2a (PREX2 Human)

Use

Tags

V5 and his

Expression

Mammalian

Mutation

Promoter

T7

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
HA-GbL

Plasmid

#1865

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

GbL (MLST8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pBGSA C5aR1-EGFP

Plasmid

#62612

Purpose

Constitutive expression of C5aR1-EGFP in mammalian cells

Depositor

Heini Miettinen

Article

Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Insert

C5aR1 (C5AR1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pCl-HA-YAP

Plasmid

#27007

Depositor

Joan Massague

Article

Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

Insert

YAP (YAP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only
pIVT-dCas9-mCherry

Plasmid

#174443

Purpose

in vitro transcription template for dCas9-mCherry

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

mCherry

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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P427-FBP_13

Plasmid

#139704

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

Plasmid

#84604

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-mRFP

Plasmid

#128126

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen (benzoate responsive promoter)

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1307

Plasmid

#111133

Purpose

Expresses WDR48.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

WDR48 (WDR48 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105 and tet

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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E[beta]C

Plasmid

#24312

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pBABE-Stat5a1*6

Plasmid

#130668

Purpose

Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

Depositor

Gerardo Ferbeyre

Article

Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

Insert

Stat5a (Stat5a Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Histidine 298 to Arginine and Serine 710 …

Promoter

LTR

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-HA

Plasmid

#79409

Purpose

Expresses C-terminally HA-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf1 (ARF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCGB

Plasmid

#135283

Purpose

GBPart - CGB Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Click Beetle Green Luciferase

Use

Luciferase and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 WT

Plasmid

#199571

Purpose

Expresses WT SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 WT (SMG1 Human)

Use

Tags

GFP fusion protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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