Addgene

We narrowed to 4,968 results for: hwa

Showing: 1521 - 1540 of 4968 results

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Article

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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N-WASP

Plasmid

#186132

Purpose

For expression of recombinant N-WASp in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

WASL (WASL Rat)

Use

Tags

His6

Expression

Bacterial

Mutation

Amino Acid residues 183-193 fused to 273-501

Promoter

T7

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Alpha1

Plasmid

#118044

Purpose

GoldenBraid2.0 Alpha 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Expression

Mutation

Promoter

8xGliBS-delta-crystallin minimal promoter

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-EGFP

Plasmid

#174442

Purpose

in vitro transcription template for dCas9-EGFP

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmCherry1

Plasmid

#31931

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

His

Expression

Bacterial

Mutation

E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

Promoter

araB

Available since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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SeeSaw Reporter (SSR2.0)

Plasmid

#50393

Purpose

SSR2.0 has two target sites in opposite orientation for generate only one cycle of repairing after I-SceI endonuclease expression.

Depositor

Pablo Huertas Sánchez

Article

Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

Insert

SSR2.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pTUP1

Plasmid

#104341

Purpose

Express TUP1

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Oct 24;283(43):28958-6

Insert

TUP1 (TUP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (1-575)

Plasmid

#1318

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (1-575) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa1-575 of Hsp104

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only

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pELuc

Plasmid

#118056

Purpose

GBPart - ELuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

ELuc

Use

Synthetic Biology; Domestication of dna parts for…

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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HA-sav

Plasmid

#32834

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Sav (SAV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
Omega 1

Plasmid

#118046

Purpose

GoldenBraid2.0 Omega 1 destination vector

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Type

Empty backbone

Use

Synthetic Biology; Assembly of gb2.0 transcriptio…

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1-1482 aa)

Plasmid

#21745

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1-1482) (Mtor Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

amino acids 1-1482

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMiniP

Plasmid

#118049

Purpose

GBPart - Minimal Promoter for expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

minimal TATA-box promoter with low basal activity

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 Y242/287A

Plasmid

#32822

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242/287A

Promoter

CMV

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pCN-mBFP

Plasmid

#63847

Purpose

Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Insert

mBFP

Use

Tags

Expression

Bacterial

Mutation

Promoter and RBS was swapped. His-tag was removed…

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SH3BP4

Plasmid

#46891

Purpose

expresses HA tagged SH3BP4 in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pET23-PBD(65-150)-N-Cys

Plasmid

#13722

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD(65-150)-N-Cys (PAK1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Met-Cys-Gly-PBD(65-150)-

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
Flag-NRF1-HA

Plasmid

#34708

Depositor

Raymond Deshaies

Article

Radhakrishnan et al Mol Cell. 2010 Apr 9;38(1):17-28.

Insert

NRF1 (NFE2L1 Human)

Use

Retroviral

Tags

3XFlag and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKe

Plasmid

#26211

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK epsilon (IKBKE Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only